Interaction trap/two-hybrid system to identify interacting proteins

Erica A. Golemis; Ilya Serebriiskii; Russell L. Finley; Mikhail G. Kolonin; Jeno Gyuris; Roger Brent

doi:10.1002/0471140864.ps1902s57

Interaction trap/two-hybrid system to identify interacting proteins

Erica A. Golemis, Ilya Serebriiskii, Russell L. Finley, Mikhail G. Kolonin, Jeno Gyuris, Roger Brent

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

Original language	English
Pages (from-to)	19.2.1-19.2.35
Journal	Current Protocols in Protein Science
Volume	Chapter 19
Issue number	SUPPL. 57
DOIs	https://doi.org/10.1002/0471140864.ps1902s57
State	Published - Jul 2009

Keywords

Animals
Cloning, Molecular
Gene Expression
Gene Library
Humans
Plasmids
Protein Binding
Proteins/genetics
Recombinant Fusion Proteins
Two-Hybrid System Techniques
Yeasts/genetics

Access to Document

10.1002/0471140864.ps1902s57

Cite this

@article{bf2e8760fed94e64ace837df4afe92dd,

title = "Interaction trap/two-hybrid system to identify interacting proteins",

abstract = "The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.",

keywords = "Animals, Cloning, Molecular, Gene Expression, Gene Library, Humans, Plasmids, Protein Binding, Proteins/genetics, Recombinant Fusion Proteins, Two-Hybrid System Techniques, Yeasts/genetics",

author = "Golemis, {Erica A.} and Ilya Serebriiskii and Finley, {Russell L.} and Kolonin, {Mikhail G.} and Jeno Gyuris and Roger Brent",

year = "2009",

month = jul,

doi = "10.1002/0471140864.ps1902s57",

language = "English",

volume = "Chapter 19",

pages = "19.2.1--19.2.35",

journal = "Current Protocols in Protein Science",

issn = "1934-3655",

publisher = "John Wiley & Sons Inc.",

number = "SUPPL. 57",

}

TY - JOUR

T1 - Interaction trap/two-hybrid system to identify interacting proteins

AU - Golemis, Erica A.

AU - Serebriiskii, Ilya

AU - Finley, Russell L.

AU - Kolonin, Mikhail G.

AU - Gyuris, Jeno

AU - Brent, Roger

PY - 2009/7

Y1 - 2009/7

N2 - The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

AB - The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

KW - Animals

KW - Cloning, Molecular

KW - Gene Expression

KW - Gene Library

KW - Humans

KW - Plasmids

KW - Protein Binding

KW - Proteins/genetics

KW - Recombinant Fusion Proteins

KW - Two-Hybrid System Techniques

KW - Yeasts/genetics

UR - http://www.scopus.com/inward/record.url?scp=69249134282&partnerID=8YFLogxK

U2 - 10.1002/0471140864.ps1902s57

DO - 10.1002/0471140864.ps1902s57

M3 - Article

C2 - 19688737

AN - SCOPUS:69249134282

SN - 1934-3655

VL - Chapter 19

SP - 19.2.1-19.2.35

JO - Current Protocols in Protein Science

JF - Current Protocols in Protein Science

IS - SUPPL. 57

ER -

Interaction trap/two-hybrid system to identify interacting proteins

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this