Interaction trap/two-hybrid system to identify interacting proteins.

Erica A. Golemis; Ilya Serebriiskii; Russell L. Finley; Mikhail G. Kolonin; Jeno Gyuris; Roger Brent

doi:10.1002/0471142727.mb2001s82

Interaction trap/two-hybrid system to identify interacting proteins.

Erica A. Golemis, Ilya Serebriiskii, Russell L. Finley, Mikhail G. Kolonin, Jeno Gyuris, Roger Brent

Fox Chase Cancer Center

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins. (c) 2008 by John Wiley & Sons, Inc.

Original language	English
Pages (from-to)	Unit 20.1
Journal	Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Volume	Chapter 20
DOIs	https://doi.org/10.1002/0471142727.mb2001s82
State	Published - Apr 2008

Keywords

Biomedical Research/methods
Peptide Library
Protein Interaction Mapping/methods
Two-Hybrid System Techniques

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AU - Finley, Russell L.

AU - Kolonin, Mikhail G.

AU - Gyuris, Jeno

AU - Brent, Roger

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AB - The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins. (c) 2008 by John Wiley & Sons, Inc.

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KW - Peptide Library

KW - Protein Interaction Mapping/methods

KW - Two-Hybrid System Techniques

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ER -

Interaction trap/two-hybrid system to identify interacting proteins.

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Keywords

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