Interaction trap/two-hybrid system to identify interacting proteins.

E. A. Golemis; I. Serebriiskii; J. Gyuris; R. Brent

Interaction trap/two-hybrid system to identify interacting proteins.

E. A. Golemis, I. Serebriiskii, J. Gyuris, R. Brent

Fox Chase Cancer Center

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.

Original language	English
Pages (from-to)	Unit 4.4
Journal	Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]
Volume	Chapter 4
State	Published - May 2001

Cite this

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title = "Interaction trap/two-hybrid system to identify interacting proteins.",

abstract = "The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.",

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T1 - Interaction trap/two-hybrid system to identify interacting proteins.

AU - Golemis, E. A.

AU - Serebriiskii, I.

AU - Gyuris, J.

AU - Brent, R.

PY - 2001/5

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N2 - The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.

AB - The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.

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M3 - Article

SN - 1934-8576

VL - Chapter 4

SP - Unit 4.4

JO - Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]

JF - Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]

ER -

Interaction trap/two-hybrid system to identify interacting proteins.

Abstract

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