Interaction trap/two-hybrid system to identify interacting proteins

Erica A. Golemis; Ilya Serebriiskii; Russel L. Finley; Mikhail G. Kolonin; Jeno Gyuris; Roger Brent

doi:10.1002/0471142727.mb2001s82

Interaction trap/two-hybrid system to identify interacting proteins

Erica A. Golemis, Ilya Serebriiskii, Russel L. Finley, Mikhail G. Kolonin, Jeno Gyuris, Roger Brent

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

Original language	English
Pages (from-to)	20.1.1-20.1.35
Journal	Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Issue number	SUPPL. 82
DOIs	https://doi.org/10.1002/0471142727.mb2001s82
State	Published - 2008

Keywords

Interaction
Interaction trap
Mating
Protein interactions
Yeast two-hybrid

Access to Document

10.1002/0471142727.mb2001s82

Cite this

@article{f04273f19db64bbdb8d8a44ea41df77f,

title = "Interaction trap/two-hybrid system to identify interacting proteins",

abstract = "The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.",

keywords = "Interaction, Interaction trap, Mating, Protein interactions, Yeast two-hybrid",

author = "Golemis, {Erica A.} and Ilya Serebriiskii and Finley, {Russel L.} and Kolonin, {Mikhail G.} and Jeno Gyuris and Roger Brent",

year = "2008",

doi = "10.1002/0471142727.mb2001s82",

language = "English",

pages = "20.1.1--20.1.35",

journal = "Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]",

issn = "1934-3639",

publisher = "John Wiley & Sons Inc.",

number = "SUPPL. 82",

}

TY - JOUR

T1 - Interaction trap/two-hybrid system to identify interacting proteins

AU - Golemis, Erica A.

AU - Serebriiskii, Ilya

AU - Finley, Russel L.

AU - Kolonin, Mikhail G.

AU - Gyuris, Jeno

AU - Brent, Roger

PY - 2008

Y1 - 2008

N2 - The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

AB - The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

KW - Interaction

KW - Interaction trap

KW - Mating

KW - Protein interactions

KW - Yeast two-hybrid

UR - http://www.scopus.com/inward/record.url?scp=57549112755&partnerID=8YFLogxK

U2 - 10.1002/0471142727.mb2001s82

DO - 10.1002/0471142727.mb2001s82

M3 - Article

SN - 1934-3639

SP - 20.1.1-20.1.35

JO - Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

JF - Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

IS - SUPPL. 82

ER -

Interaction trap/two-hybrid system to identify interacting proteins

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this