Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA

David Arnot; James W. Lillie; Charles Auffray; Dietmar Kappes; Jack L. Strominger

doi:10.1007/BF00364206

Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA

David Arnot, James W. Lillie, Charles Auffray, Dietmar Kappes, Jack L. Strominger

Harvard University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We have constructed cDNA clone libraries from two lymphoblastoid cell lines, JY (HLA-A2, B7, C untypeable) and LB (HLA-A28, B40, Cw3), and isolated clones encoding class I HLA antigens. We have characterized short oligonucleotide probes derived from the coding region of the HLA class I antigens which are specific for the HLA-A and -B loci. These probes have been used to subdivide the class I cDNA clones into subclasses. DNA sequencing of several HLA-A and -B related clones has allowed us to extend the primary structural characterization of these cell-surface antigens. This analysis has also detected a sequence polymorphism at the HLA-A locus, indicating that the previously considered homozygous typing cell line LB expresses two alleles of similar, although not identical, serological specificity.

Original language	English
Pages (from-to)	237-252
Number of pages	16
Journal	Immunogenetics
Volume	20
Issue number	3
DOIs	https://doi.org/10.1007/BF00364206
State	Published - Sep 1984

Keywords

Alleles
Amino Acid Sequence
B-Lymphocytes/immunology
Base Sequence
Cell Line
Cloning, Molecular
DNA/metabolism
Genes
HLA Antigens/genetics
Humans
Nucleic Acid Hybridization
Plasmids
Polymorphism, Genetic
RNA, Messenger/genetics

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10.1007/BF00364206

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title = "Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA",

abstract = "We have constructed cDNA clone libraries from two lymphoblastoid cell lines, JY (HLA-A2, B7, C untypeable) and LB (HLA-A28, B40, Cw3), and isolated clones encoding class I HLA antigens. We have characterized short oligonucleotide probes derived from the coding region of the HLA class I antigens which are specific for the HLA-A and -B loci. These probes have been used to subdivide the class I cDNA clones into subclasses. DNA sequencing of several HLA-A and -B related clones has allowed us to extend the primary structural characterization of these cell-surface antigens. This analysis has also detected a sequence polymorphism at the HLA-A locus, indicating that the previously considered homozygous typing cell line LB expresses two alleles of similar, although not identical, serological specificity.",

keywords = "Alleles, Amino Acid Sequence, B-Lymphocytes/immunology, Base Sequence, Cell Line, Cloning, Molecular, DNA/metabolism, Genes, HLA Antigens/genetics, Humans, Nucleic Acid Hybridization, Plasmids, Polymorphism, Genetic, RNA, Messenger/genetics",

author = "David Arnot and Lillie, {James W.} and Charles Auffray and Dietmar Kappes and Strominger, {Jack L.}",

year = "1984",

month = sep,

doi = "10.1007/BF00364206",

language = "English",

volume = "20",

pages = "237--252",

journal = "Immunogenetics",

issn = "0093-7711",

publisher = "Springer Verlag",

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T1 - Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA

AU - Arnot, David

AU - Lillie, James W.

AU - Auffray, Charles

AU - Kappes, Dietmar

AU - Strominger, Jack L.

PY - 1984/9

Y1 - 1984/9

N2 - We have constructed cDNA clone libraries from two lymphoblastoid cell lines, JY (HLA-A2, B7, C untypeable) and LB (HLA-A28, B40, Cw3), and isolated clones encoding class I HLA antigens. We have characterized short oligonucleotide probes derived from the coding region of the HLA class I antigens which are specific for the HLA-A and -B loci. These probes have been used to subdivide the class I cDNA clones into subclasses. DNA sequencing of several HLA-A and -B related clones has allowed us to extend the primary structural characterization of these cell-surface antigens. This analysis has also detected a sequence polymorphism at the HLA-A locus, indicating that the previously considered homozygous typing cell line LB expresses two alleles of similar, although not identical, serological specificity.

AB - We have constructed cDNA clone libraries from two lymphoblastoid cell lines, JY (HLA-A2, B7, C untypeable) and LB (HLA-A28, B40, Cw3), and isolated clones encoding class I HLA antigens. We have characterized short oligonucleotide probes derived from the coding region of the HLA class I antigens which are specific for the HLA-A and -B loci. These probes have been used to subdivide the class I cDNA clones into subclasses. DNA sequencing of several HLA-A and -B related clones has allowed us to extend the primary structural characterization of these cell-surface antigens. This analysis has also detected a sequence polymorphism at the HLA-A locus, indicating that the previously considered homozygous typing cell line LB expresses two alleles of similar, although not identical, serological specificity.

KW - Alleles

KW - Amino Acid Sequence

KW - B-Lymphocytes/immunology

KW - Base Sequence

KW - Cell Line

KW - Cloning, Molecular

KW - DNA/metabolism

KW - Genes

KW - HLA Antigens/genetics

KW - Humans

KW - Nucleic Acid Hybridization

KW - Plasmids

KW - Polymorphism, Genetic

KW - RNA, Messenger/genetics

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U2 - 10.1007/BF00364206

DO - 10.1007/BF00364206

M3 - Article

C2 - 6332068

AN - SCOPUS:0021266473

SN - 0093-7711

VL - 20

SP - 237

EP - 252

JO - Immunogenetics

JF - Immunogenetics

IS - 3

ER -

Inter-locus and intra-allelic polymorphisms of HLA class I antigen gene mRNA

Abstract

Keywords

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