Abstract
To assess the importance of monoclonal antibody route of administration, we compared the selective (intraportal) and systemic injection of specific radiolabeled monoclonal antibody, using a murine model of hepatic metastases from a human colorectal carcinoma. Tumor uptake was studied over time after injection of 0.1, 1.0 or 2.0 micrograms of a specific antibody (HT29-15) or an isotype-matched control (BL-3). Significantly higher tumor uptake and tumor/liver or tumor/blood uptake ratios were seen in animals receiving intraportal injection for all tested doses. Intraportal injection of specific monoclonal antibody resulted in significant improvement in metastases uptake; these findings could be applied to the diagnosis and treatment of hepatic metastases from colorectal cancer using radiolabeled monoclonal antibodies.
| Translated title of the contribution | Intraportal injection of specific monoclonal antibody in nude mice bearing hepatic metastases |
|---|---|
| Original language | French |
| Pages (from-to) | 721-729 |
| Number of pages | 9 |
| Journal | Journal de Chirurgie Viscerale |
| Volume | 116 |
| Issue number | 8-9 |
| State | Published - 1990 |
Keywords
- Adenocarcinoma
- Animals
- Antibodies, Monoclonal/administration & dosage
- Clinical Protocols
- Colonic Neoplasms
- Female
- Injections, Intravenous
- Liver Neoplasms/metabolism
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Transplantation
- Portal Vein
- Research Design