TY - GEN
T1 - Inflammation precedes the development of human malignant mesotheliomas in a SCID mouse xenograft model
AU - Hillegass, Jedd M.
AU - Shukla, Arti
AU - Lathrop, Sherrill A.
AU - MacPherson, Maximilian B.
AU - Beuschel, Stacie L.
AU - Butnor, Kelly J.
AU - Testa, Joseph R.
AU - Pass, Harvey I.
AU - Carbone, Michele
AU - Steele, Chad
AU - Mossman, Brooke T.
PY - 2010/8
Y1 - 2010/8
N2 - Asbestos fibers cause chronic inflammation that may be critical to the development of malignant mesothelioma (MM). Two human MM cell lines (Hmeso, PPM Mill) were used in a SCID mouse xenograft model to assess time-dependent patterns of inflammation and tumor formation. After intraperitoneal (IP) injection of MM cells, mice were euthanized at 7, 14, and 30 days, and peritoneal lavage fluid (PLF) was examined for immune cell profiles and human and mouse cytokines. Increases in human MM-derived IL-6, IL-8, bFGF, and VEGF were observed in mice at 7 days postinjection of either MM line, and a striking neutrophilia was observed at all time points. Free-floating tumor spheroids developed in mice at 14 days, and both spheroids and adherent MM tumor masses occurred in all mice at 30 days. Results suggest that inflammation and cytokine production precede and may be critical to the development of MMs.
AB - Asbestos fibers cause chronic inflammation that may be critical to the development of malignant mesothelioma (MM). Two human MM cell lines (Hmeso, PPM Mill) were used in a SCID mouse xenograft model to assess time-dependent patterns of inflammation and tumor formation. After intraperitoneal (IP) injection of MM cells, mice were euthanized at 7, 14, and 30 days, and peritoneal lavage fluid (PLF) was examined for immune cell profiles and human and mouse cytokines. Increases in human MM-derived IL-6, IL-8, bFGF, and VEGF were observed in mice at 7 days postinjection of either MM line, and a striking neutrophilia was observed at all time points. Free-floating tumor spheroids developed in mice at 14 days, and both spheroids and adherent MM tumor masses occurred in all mice at 30 days. Results suggest that inflammation and cytokine production precede and may be critical to the development of MMs.
KW - Animals
KW - Carcinoma/chemistry
KW - Cell Line
KW - Cell Line, Tumor
KW - Cell Transformation, Neoplastic/chemistry
KW - Cytokines/biosynthesis
KW - Disease Models, Animal
KW - Fibrosarcoma/chemistry
KW - Humans
KW - Inflammation Mediators/chemistry
KW - Intercellular Signaling Peptides and Proteins/biosynthesis
KW - Male
KW - Mesothelioma/chemistry
KW - Mice
KW - Mice, SCID
KW - Neutrophils/pathology
KW - Pleural Neoplasms/chemistry
KW - Protein Array Analysis
KW - Time Factors
KW - Xenograft Model Antitumor Assays/methods
UR - https://www.scopus.com/pages/publications/77956247365
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000287380600002&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1111/j.1749-6632.2010.05554.x
DO - 10.1111/j.1749-6632.2010.05554.x
M3 - Conference contribution
C2 - 20716277
SN - 9781573317849
VL - 1203
T3 - Annals of the New York Academy of Sciences
SP - 7
EP - 14
BT - Oxidative/Nitrosative Stress and Disease
PB - Blackwell Publishing Inc.
ER -