Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Beata Kosmider; Elzbieta Zyner; Regina Osiecka; Justyn Ochocki

doi:10.1016/j.mrgentox.2004.05.018

Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Beata Kosmider
, Elzbieta Zyner
, Regina Osiecka
, Justyn Ochocki

Cancer Prevention and Control (CPC)

University of Lodz
Texas A&M University
Medical University of Łódź

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.

Original language	English
Pages (from-to)	61-70
Number of pages	10
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	563
Issue number	1
DOIs	https://doi.org/10.1016/j.mrgentox.2004.05.018
State	Published - Sep 12 2004

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acridine Orange/metabolism
Antineoplastic Agents/chemistry
Apoptosis
Cell Division
Cell Line, Tumor
Cell Survival
Cisplatin/chemistry
Ethidium/metabolism
Flavonoids/chemistry
Fluorescent Dyes/metabolism
Humans
In Situ Nick-End Labeling
Molecular Structure
Necrosis
Tetrazolium Salts/metabolism
Thiazoles/metabolism

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10.1016/j.mrgentox.2004.05.018

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title = "Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP",

abstract = "Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.",

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author = "Beata Kosmider and Elzbieta Zyner and Regina Osiecka and Justyn Ochocki",

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AU - Kosmider, Beata

AU - Zyner, Elzbieta

AU - Osiecka, Regina

AU - Ochocki, Justyn

PY - 2004/9/12

Y1 - 2004/9/12

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AB - Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.

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KW - Cell Division

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KW - Cell Survival

KW - Cisplatin/chemistry

KW - Ethidium/metabolism

KW - Flavonoids/chemistry

KW - Fluorescent Dyes/metabolism

KW - Humans

KW - In Situ Nick-End Labeling

KW - Molecular Structure

KW - Necrosis

KW - Tetrazolium Salts/metabolism

KW - Thiazoles/metabolism

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SN - 1383-5718

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JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

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IS - 1

ER -

Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Abstract

UN SDGs

Keywords

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