Abstract
Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.
Original language | English |
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Pages (from-to) | 61-70 |
Number of pages | 10 |
Journal | Mutation Research - Genetic Toxicology and Environmental Mutagenesis |
Volume | 563 |
Issue number | 1 |
DOIs | |
State | Published - Sep 12 2004 |
Keywords
- Acridine Orange/metabolism
- Antineoplastic Agents/chemistry
- Apoptosis
- Cell Division
- Cell Line, Tumor
- Cell Survival
- Cisplatin/chemistry
- Ethidium/metabolism
- Flavonoids/chemistry
- Fluorescent Dyes/metabolism
- Humans
- In Situ Nick-End Labeling
- Molecular Structure
- Necrosis
- Tetrazolium Salts/metabolism
- Thiazoles/metabolism