Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP

Beata Kosmider, Elzbieta Zyner, Regina Osiecka, Justyn Ochocki

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Non-small cell lung cancer (NSCLC) includes a group of tumors that respond poorly to drugs. cis-Diamminedichloroplatinum(II) (cis-DDP) toxicity still remains problematic, and not completely solved by the improvement of supportive care. Therefore, the cis-Pt(II) complex of 3-aminoflavone was selected from cis-DDP analogues with a more favourable toxic profile towards normal cells and at least similar or better anti-tumor activity in comparison with cis-DDP. The aim of this research is to compare the ability of the cis-Pt(II) complex of 3-aminoflavone and cis-DDP to induce apoptosis and necrosis in the human non-small cancer cell line A549. Trypan blue dye exclusion, fluorochrome staining (acridine orange/ethidium bromide double staining), MTT and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used. The results obtained show that the cis-Pt(II) complex of 3-aminoflavone is more active in inducing apoptosis and necrosis and in decreasing viability in A549 cells than cis-DDP, which suggests that it could be a potential chemotherapeutic drug.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume563
Issue number1
DOIs
StatePublished - Sep 12 2004

Keywords

  • Acridine Orange/metabolism
  • Antineoplastic Agents/chemistry
  • Apoptosis
  • Cell Division
  • Cell Line, Tumor
  • Cell Survival
  • Cisplatin/chemistry
  • Ethidium/metabolism
  • Flavonoids/chemistry
  • Fluorescent Dyes/metabolism
  • Humans
  • In Situ Nick-End Labeling
  • Molecular Structure
  • Necrosis
  • Tetrazolium Salts/metabolism
  • Thiazoles/metabolism

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