TY - JOUR
T1 - Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: A novel system for screening potential inhibitors of HBV replication
AU - Ladner, Stephanie K.
AU - Otto, Michael J.
AU - Barker, Christopher S.
AU - Zaifert, Katie
AU - Wang, Guang Hua
AU - Guo, J. U.Tao
AU - Seeger, Christoph
AU - King, Robert W.
N1 - Times Cited: 19 English Article XP605 ANTIMICROB AGENTS CHEMOTHER
PY - 1997/8
Y1 - 1997/8
N2 - We report the development and isolation of a cell line, termed HepAD38, that replicates human hepatitis B virus (HBV) under conditions that can be regulated with tetracycline. In the presence of the antibiotic, this cell line is free of virus due to the repression of pregenomic (pg) RNA synthesis, Upon removal of tetracycline from the culture medium, the cells express viral pg RNA, accumulate subviral particles in the cytoplasm that contain DNA intermediates characteristic of viral replication, and secrete virus-like particles Into the supernatant. Since the HepAD38 cell line can produce high levels of HBV DNA, it should be useful for analyses of the viral replication cycle that depend upon viral DNA synthesis in a synchronized fashion, In addition, this cell line has been formatted into a high-throughput, cell-based assay that permits the large-scale screening of diverse compound libraries for new classes of inhibitors of HBV replication.
AB - We report the development and isolation of a cell line, termed HepAD38, that replicates human hepatitis B virus (HBV) under conditions that can be regulated with tetracycline. In the presence of the antibiotic, this cell line is free of virus due to the repression of pregenomic (pg) RNA synthesis, Upon removal of tetracycline from the culture medium, the cells express viral pg RNA, accumulate subviral particles in the cytoplasm that contain DNA intermediates characteristic of viral replication, and secrete virus-like particles Into the supernatant. Since the HepAD38 cell line can produce high levels of HBV DNA, it should be useful for analyses of the viral replication cycle that depend upon viral DNA synthesis in a synchronized fashion, In addition, this cell line has been formatted into a high-throughput, cell-based assay that permits the large-scale screening of diverse compound libraries for new classes of inhibitors of HBV replication.
KW - Cell Line
KW - DNA, Viral/analysis
KW - Hepatitis B virus/drug effects
KW - Hepatoblastoma/pathology
KW - Humans
KW - Liver Neoplasms/pathology
KW - Protein Synthesis Inhibitors/pharmacology
KW - RNA, Viral/analysis
KW - Tetracycline/pharmacology
KW - Transfection
KW - Tumor Cells, Cultured/pathology
KW - Virus Replication/drug effects
UR - http://www.scopus.com/inward/record.url?scp=0030842540&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1997XP60500016&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1128/aac.41.8.1715
DO - 10.1128/aac.41.8.1715
M3 - Article
C2 - 9257747
SN - 0066-4804
VL - 41
SP - 1715
EP - 1720
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 8
ER -
