TY - JOUR
T1 - Incomplete endoplasmic reticulum (ER) retention in immature thymocytes as revealed by surface expression of "ER-resident" molecular chaperones
AU - Wiest, David L.
AU - Bhandoola, Avinash
AU - Punt, Jennifer
AU - Kreibich, Gert
AU - Mckean, David
AU - Singer, Alfred
PY - 1997/3/4
Y1 - 1997/3/4
N2 - The folding and assembly of nascent proteins in the endoplasmic reticulum (ER) is assisted by molecular chaperones that are themselves retained within the ER. We now report that a number of different ER proteins, including molecular chaperones, are selectively expressed on the surface of immature thymocytes, but their surface expression is extinguished upon further differentiation. Escape from the ER is only possible for newly synthesized ER proteins before they become permanently retained. Thus, the cellular process of ER retention is incomplete in immature thymocytes and provides an explanation for surface expression of partial receptor complexes that transduce differentiative signals during thymic development.
AB - The folding and assembly of nascent proteins in the endoplasmic reticulum (ER) is assisted by molecular chaperones that are themselves retained within the ER. We now report that a number of different ER proteins, including molecular chaperones, are selectively expressed on the surface of immature thymocytes, but their surface expression is extinguished upon further differentiation. Escape from the ER is only possible for newly synthesized ER proteins before they become permanently retained. Thus, the cellular process of ER retention is incomplete in immature thymocytes and provides an explanation for surface expression of partial receptor complexes that transduce differentiative signals during thymic development.
KW - Blotting, Western
KW - CD3 Complex/metabolism
KW - Calcium-Binding Proteins/metabolism
KW - Calnexin
KW - Cell Differentiation
KW - Cell Membrane/metabolism
KW - Cells, Cultured
KW - Endoplasmic Reticulum/chemistry
KW - Flow Cytometry
KW - Gene Expression Regulation, Developmental/genetics
KW - Humans
KW - Membrane Proteins/metabolism
KW - Molecular Chaperones/metabolism
KW - Precipitin Tests
KW - Protein Folding
KW - T-Lymphocytes/metabolism
KW - Thymus Gland/cytology
UR - http://www.scopus.com/inward/record.url?scp=0031055601&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1997WM05900054&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1073/pnas.94.5.1884
DO - 10.1073/pnas.94.5.1884
M3 - Article
C2 - 9050874
SN - 0027-8424
VL - 94
SP - 1884
EP - 1889
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 5
ER -