TY - JOUR
T1 - Immunohistologic Study of Ulcerative Colitis With Monoclonal Antibodies Against Tumor-Associated and/or Differentiation Antigens
AU - Cooper, Harry S.
AU - Steplewski, Zenon
PY - 1988
Y1 - 1988
N2 - We used monoclonal antibodies (MAbs) (19-9, 55-2, and 73-3) that detect tumor-associated or differentiation antigens, or both, to immunohistochemically study a well-defined group of patients with ulcerative colitis. Monoclonal antibody 19-9 detects the gastrointestinal cancer-associated antigen (sialylated Lewis A). Monoclonal antibody 55-2 detects the Lewis Y antigen and reacts with deep crypt cells in the normal colon. In the normal colon MAb 73-3 reacts with mature superficial columnar cells detecting the protein moiety of a 35,000-dalton glycoprotein. In cases of inactive or mildly active disease, MAbs 19-9, 55-2, and 73-3 had staining patterns similar to normal colon. In 72% and 44% of cases of severely active disease, MAb 19-9 and MAb 73-3, respectively, reacted with epithelial cells at all levels of the crypt, whereas MAb 55-2 reacted only with deep crypt cells. Monoclonal antibodies 19-9, 55-2, and 73-3 reacted with dysplastic epithelium in 70%, 10%, and 60% of cases, respectively. In severely active disease, proliferating epithelial cells “paradoxically“ express markers of differentiated epithelium throughout the entire crypt. Similarly, colonic epithelial cells may have the ability to reversibly express tumor-associated antigens. Unfortunately, the MAbs used in this study cannot differentiate dysplasia from reactive epithelium.
AB - We used monoclonal antibodies (MAbs) (19-9, 55-2, and 73-3) that detect tumor-associated or differentiation antigens, or both, to immunohistochemically study a well-defined group of patients with ulcerative colitis. Monoclonal antibody 19-9 detects the gastrointestinal cancer-associated antigen (sialylated Lewis A). Monoclonal antibody 55-2 detects the Lewis Y antigen and reacts with deep crypt cells in the normal colon. In the normal colon MAb 73-3 reacts with mature superficial columnar cells detecting the protein moiety of a 35,000-dalton glycoprotein. In cases of inactive or mildly active disease, MAbs 19-9, 55-2, and 73-3 had staining patterns similar to normal colon. In 72% and 44% of cases of severely active disease, MAb 19-9 and MAb 73-3, respectively, reacted with epithelial cells at all levels of the crypt, whereas MAb 55-2 reacted only with deep crypt cells. Monoclonal antibodies 19-9, 55-2, and 73-3 reacted with dysplastic epithelium in 70%, 10%, and 60% of cases, respectively. In severely active disease, proliferating epithelial cells “paradoxically“ express markers of differentiated epithelium throughout the entire crypt. Similarly, colonic epithelial cells may have the ability to reversibly express tumor-associated antigens. Unfortunately, the MAbs used in this study cannot differentiate dysplasia from reactive epithelium.
KW - Antibodies, Monoclonal
KW - Antigens, Differentiation/analysis
KW - Antigens, Neoplasm/analysis
KW - Antigens, Tumor-Associated, Carbohydrate
KW - Colitis, Ulcerative/immunology
KW - Colon/immunology
KW - Humans
KW - Immunohistochemistry
KW - Isoantigens/immunology
KW - Lewis Blood Group Antigens/immunology
UR - http://www.scopus.com/inward/record.url?scp=0023729350&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1988P656800015&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1016/S0016-5085(88)80015-5
DO - 10.1016/S0016-5085(88)80015-5
M3 - Article
C2 - 3165075
SN - 0016-5085
VL - 95
SP - 686
EP - 693
JO - Gastroenterology
JF - Gastroenterology
IS - 3
ER -