Immunohistologic Study of Ulcerative Colitis With Monoclonal Antibodies Against Tumor-Associated and/or Differentiation Antigens

Harry S. Cooper, Zenon Steplewski

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

We used monoclonal antibodies (MAbs) (19-9, 55-2, and 73-3) that detect tumor-associated or differentiation antigens, or both, to immunohistochemically study a well-defined group of patients with ulcerative colitis. Monoclonal antibody 19-9 detects the gastrointestinal cancer-associated antigen (sialylated Lewis A). Monoclonal antibody 55-2 detects the Lewis Y antigen and reacts with deep crypt cells in the normal colon. In the normal colon MAb 73-3 reacts with mature superficial columnar cells detecting the protein moiety of a 35,000-dalton glycoprotein. In cases of inactive or mildly active disease, MAbs 19-9, 55-2, and 73-3 had staining patterns similar to normal colon. In 72% and 44% of cases of severely active disease, MAb 19-9 and MAb 73-3, respectively, reacted with epithelial cells at all levels of the crypt, whereas MAb 55-2 reacted only with deep crypt cells. Monoclonal antibodies 19-9, 55-2, and 73-3 reacted with dysplastic epithelium in 70%, 10%, and 60% of cases, respectively. In severely active disease, proliferating epithelial cells “paradoxically“ express markers of differentiated epithelium throughout the entire crypt. Similarly, colonic epithelial cells may have the ability to reversibly express tumor-associated antigens. Unfortunately, the MAbs used in this study cannot differentiate dysplasia from reactive epithelium.

Original languageEnglish
Pages (from-to)686-693
Number of pages8
JournalGastroenterology
Volume95
Issue number3
DOIs
StatePublished - 1988

Keywords

  • Antibodies, Monoclonal
  • Antigens, Differentiation/analysis
  • Antigens, Neoplasm/analysis
  • Antigens, Tumor-Associated, Carbohydrate
  • Colitis, Ulcerative/immunology
  • Colon/immunology
  • Humans
  • Immunohistochemistry
  • Isoantigens/immunology
  • Lewis Blood Group Antigens/immunology

Fingerprint

Dive into the research topics of 'Immunohistologic Study of Ulcerative Colitis With Monoclonal Antibodies Against Tumor-Associated and/or Differentiation Antigens'. Together they form a unique fingerprint.

Cite this