Identification of Cryptosporidium in paraffin-embedded tissue sections with the use of a monoclonal antibody

J. H. Loose, D. J. Sedergran, H. S. Cooper

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Recently a monoclonal antibody has been developed against the oocyst form of Cryptosporidium species for use in detecting the organism in fecal material. The authors undertook this study to determine if this antibody could be used in identifying Cryptosporidium species in paraffin-embedded tissue sections. Three biopsies showing Cryptosporidiosis as diagnosed by characteristic appearance by light and electron microscopy were selected from the surgical pathology files at the authors' institution. Paraffin sections were examined with the use of both an indirect immunofluorescence and an avidin-biotin-peroxidase technique. In all the cases, prominent staining of oocysts was seen; however, intracellular trophozoites did not react with the antibody. The authors conclude that this antibody can be used to identify Cryptosporidium species oocysts in tissue sections and that the antibody appears to be specific for the oocyst form only.

Original languageEnglish
Pages (from-to)206-209
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume91
Issue number2
DOIs
StatePublished - 1989

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Antibodies, Monoclonal
  • Coccidia/isolation & purification
  • Cryptosporidium/isolation & purification
  • Duodenum/microbiology
  • Feces/microbiology
  • Female
  • Fluorescent Antibody Technique
  • Histological Techniques
  • Humans
  • Immunoenzyme Techniques
  • Male

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