Identification of Akt interaction protein PHF20/TZP that transcriptionally regulates p53

Sungman Park, Donghwa Kim, Han C. Dan, Huihua Chen, Joseph R. Testa, Jin Q. Cheng

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Akt regulates a diverse array of cellular functions, including cell survival, proliferation, differentiation, and metabolism. Although a number of molecules have been identified as upstream regulators and downstream targets of Akt, the mechanisms by which Akt regulates these cellular processes remain elusive. Here, we demonstrate that a novel transcription factor, PHF20/TZP (referring to Tudor and zinc finger domain containing protein), binds to Akt and induces p53 expression at the transcription level. Knockdown of PHF20 significantly reduces p53. PHF20 inhibits cell growth, DNA synthesis, and cell survival. Akt phosphorylates PHF20 at Ser291 in vitro and in vivo, which results in its translocation from the nucleus to the cytoplasm and attenuation of PHF20 function. These data indicate that PHF20 is a substrate of Akt and plays a role in Akt cell survival/growth signaling.

Original languageEnglish
Pages (from-to)11151-11163
Number of pages13
JournalJournal of Biological Chemistry
Volume287
Issue number14
DOIs
StatePublished - Mar 30 2012

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