TY - JOUR
T1 - ID1 promotes breast cancer metastasis by S100A9 regulation
AU - Gumireddy, Kiranmai
AU - Li, Anping
AU - Kossenkov, Andrew V.
AU - Cai, Kathy Q.
AU - Liu, Qin
AU - Yan, Jinchun
AU - Xu, Hua
AU - Showe, Louise
AU - Zhang, Lin
AU - Huang, Qihong
N1 - Publisher Copyright:
© 2014 American Association for Cancer Research.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Metastasis is a major factor responsible for mortality in patients with breast cancer. Inhibitor of DNA binding 1 (Id1) has been shown to play an important role in cell differentiation, tumor angiogenesis, cell invasion, and metastasis. Despite the data establishing Id1 as a critical factor for lung metastasis in breast cancer, the pathways and molecular mechanisms of Id1 functions in metastasis remain to be defined. Here, we show that Id1 interacts with TFAP2A to suppress S100A9 expression. We show that expression of Id1 and S100A9 is inversely correlated in both breast cancer cell lines and clinical samples. We also show that the migratory and invasive phenotypes in vitro and metastasis in vivo induced by Id1 expression are rescued by reestablishment of S100A9 expression. S100A9 also suppresses the expression of known metastasis-promoting factor RhoC activated by Id1 expression. Our results suggest that Id1 promotes breast cancer metastasis by the suppression of S100A9 expression.
AB - Metastasis is a major factor responsible for mortality in patients with breast cancer. Inhibitor of DNA binding 1 (Id1) has been shown to play an important role in cell differentiation, tumor angiogenesis, cell invasion, and metastasis. Despite the data establishing Id1 as a critical factor for lung metastasis in breast cancer, the pathways and molecular mechanisms of Id1 functions in metastasis remain to be defined. Here, we show that Id1 interacts with TFAP2A to suppress S100A9 expression. We show that expression of Id1 and S100A9 is inversely correlated in both breast cancer cell lines and clinical samples. We also show that the migratory and invasive phenotypes in vitro and metastasis in vivo induced by Id1 expression are rescued by reestablishment of S100A9 expression. S100A9 also suppresses the expression of known metastasis-promoting factor RhoC activated by Id1 expression. Our results suggest that Id1 promotes breast cancer metastasis by the suppression of S100A9 expression.
KW - Breast Neoplasms/genetics
KW - Calgranulin B/biosynthesis
KW - Cell Differentiation/genetics
KW - Cell Line, Tumor
KW - Cell Proliferation/genetics
KW - Female
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Inhibitor of Differentiation Protein 1/genetics
KW - Neoplasm Metastasis
KW - Neovascularization, Pathologic/genetics
KW - RNA, Small Interfering
KW - Signal Transduction
UR - http://www.scopus.com/inward/record.url?scp=84907280014&partnerID=8YFLogxK
U2 - 10.1158/1541-7786.MCR-14-0049
DO - 10.1158/1541-7786.MCR-14-0049
M3 - Article
C2 - 24948111
AN - SCOPUS:84907280014
SN - 1541-7786
VL - 12
SP - 1334
EP - 1343
JO - Molecular Cancer Research
JF - Molecular Cancer Research
IS - 9
ER -
