TY - JOUR
T1 - Hyperplastic polyps of the colon and rectum. An immunohistochemical study with monoclonal antibodies against blood groups antigens (Sialosyl-Lea, Leb, Le(x), Le(y), A, B, H)
AU - Cooper, H. S.
AU - Marshall, C.
AU - Ruggerio, F.
AU - Steplewski, Z.
PY - 1987
Y1 - 1987
N2 - We studied 40 hyperplastic polyps (HP) immunohistochemically with monoclonal antibodies against 8 different blood group antigens (BGA) comparing their reactivity with normal control colon and colorectal adenocarcinomas. The 8 BGA studied were: Sialosyl-Le
a, Le
a, Le
b, Le(x), Le(y), A, B, and H. Sialosyl-Le
a, Le
a, Le(x), and Le(y) can be thought of as differentiation antigens. The former 2 BGA are expressed on mature (differentiated) epithelium while the latter 2 BGA are expressed by undifferentiated epithelium of the crypt base. A, B, H and Le
b are not expressed in the normal distal colon, however, they are extenively expressed on distal colorectal cancers and andenomas and can be considered onocofetal BGA. HP expressed Le
a, Le(x), Le(y) in the same compartment of the crypt as normal colon and extensively expressed Sialosyl-Le
a throughout the entire length of the crypt. This latter finding indicated maturation at a lower point in the crypt than in normals. All HP failed to express B and H BGA, while 6 of 40 expressed Le
b and 5 of 50 HP expressed A BGA. Of the 6 HP expressing Le
b BGA, 3 were from patients with synchronous or metachronous cancers and 2 from patients with mixed hyperplastic polyp-adenomas (HP/AD). Two of the HP expressing A BGA were from patients with HP/AD. The expression and distribution of these BGA in HP, especially the extensive expression of sialosyl-Le
a correlates with the known cell kinetics of HP. While nonneoplastic in nature, HP may occasionally express true oncofetal BGA. Similarly, the HP component of HP/AD may also express true onocofetal BGA. These data suggest that the lesions classified morphologically as HP may be antigenically heterogenous.
AB - We studied 40 hyperplastic polyps (HP) immunohistochemically with monoclonal antibodies against 8 different blood group antigens (BGA) comparing their reactivity with normal control colon and colorectal adenocarcinomas. The 8 BGA studied were: Sialosyl-Le
a, Le
a, Le
b, Le(x), Le(y), A, B, and H. Sialosyl-Le
a, Le
a, Le(x), and Le(y) can be thought of as differentiation antigens. The former 2 BGA are expressed on mature (differentiated) epithelium while the latter 2 BGA are expressed by undifferentiated epithelium of the crypt base. A, B, H and Le
b are not expressed in the normal distal colon, however, they are extenively expressed on distal colorectal cancers and andenomas and can be considered onocofetal BGA. HP expressed Le
a, Le(x), Le(y) in the same compartment of the crypt as normal colon and extensively expressed Sialosyl-Le
a throughout the entire length of the crypt. This latter finding indicated maturation at a lower point in the crypt than in normals. All HP failed to express B and H BGA, while 6 of 40 expressed Le
b and 5 of 50 HP expressed A BGA. Of the 6 HP expressing Le
b BGA, 3 were from patients with synchronous or metachronous cancers and 2 from patients with mixed hyperplastic polyp-adenomas (HP/AD). Two of the HP expressing A BGA were from patients with HP/AD. The expression and distribution of these BGA in HP, especially the extensive expression of sialosyl-Le
a correlates with the known cell kinetics of HP. While nonneoplastic in nature, HP may occasionally express true oncofetal BGA. Similarly, the HP component of HP/AD may also express true onocofetal BGA. These data suggest that the lesions classified morphologically as HP may be antigenically heterogenous.
KW - ABO Blood-Group System
KW - Adenocarcinoma/immunology
KW - Antibodies, Monoclonal
KW - Antigens, Differentiation/analysis
KW - Colonic Neoplasms/immunology
KW - Epithelium/immunology
KW - Humans
KW - Immunoenzyme Techniques
KW - Intestinal Polyps/immunology
KW - Lewis Blood Group Antigens/immunology
KW - Rectal Neoplasms/immunology
KW - Tissue Distribution
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M3 - Article
C2 - 3312809
SN - 0023-6837
VL - 57
SP - 421
EP - 428
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 4
ER -