Abstract
The heat shock protein Hsp90 is known as an essential component of several signal transduction pathways and has now been identified as an essential host factor for hepatitis B virus replication. Hsp90 interacts with the viral reverse transcriptase to facilitate the formation of a ribonucleoprotein (RNP) complex between the polymerase and an RNA ligand. This RNP complex is required early in replication for viral assembly and initiation of DNA synthesis through a protein-priming mechanism. These results thus invoke a role for the Hsp90 pathway in the formation of an RNP.
| Original language | English |
|---|---|
| Pages (from-to) | 1060-1064 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 93 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 6 1996 |
Keywords
- Animals
- Benzoquinones
- Carcinoma, Hepatocellular
- Cell Line
- Chickens
- DNA Replication
- Enzyme Inhibitors/pharmacology
- HSP90 Heat-Shock Proteins/isolation & purification
- Hepatitis B virus/enzymology
- Kinetics
- Lactams, Macrocyclic
- Liver Neoplasms
- Models, Genetic
- Quinones/pharmacology
- RNA, Viral/metabolism
- RNA-Directed DNA Polymerase/biosynthesis
- Recombinant Proteins/biosynthesis
- Ribonucleoproteins/isolation & purification
- Signal Transduction
- Transfection
- Tumor Cells, Cultured
- Virus Replication
