TY - JOUR
T1 - GTSF-2
T2 - A new, versatile cell culture medium for diverse normal and transformed mammalian cells
AU - Lelkes, Peter I.
AU - Ramos, Esther M.
AU - Nikolaychik, V. V.
AU - Wankowski, Dawn M.
AU - Unsworth, B. R.
AU - Goodwin, Thomas J.
PY - 1997/5
Y1 - 1997/5
N2 - The aim of this study was to test the versatility of a new basal cell culture medium, GTSF-2. In addition to traditional growth-factors, GTSF-2 contains a blend of three sugars (glucose, galactose and fructose) at their physiological levels. For these studies, we isolated normal endothelial cells from human, bovine, and rat large blood vessels and microvessels. In addition, GTSF-2 was also tested as a replacement for high-glucose-containing medium for PC12 pheochromocytoma cells and for other, transformed cell lines. The cell growth characteristics were assessed with a novel cell viability and proliferation assay, which is based on the bioreduction of the fluorescent dye, Alamar Blue. After appropriate calibration, the Alamar Blue assay was found to be equivalent to established cell proliferation assays. Alamar Blue offers the advantage that cell proliferation can be measured in the same wells over an extended period of time. For some of the cell types (e.g., endothelial cells isolated from the bovine aorta, the rat adrenal medulla, or the transformed cells), proliferation in unmodified GTSF-2 was equivalent to that in the original culture media. For others cell types (e.g., human umbilical vein endothelial cells and PC12 cells), GTSF-2 proved to be a superior growth medium, when supplemented with simple additives, such as endothelial cells growth supplement (bFGF) or horse serum. Our results suggest that GTSF-2 is a versatile basal medium that will be useful for studying organ-specific in heterotypic coculture studies.
AB - The aim of this study was to test the versatility of a new basal cell culture medium, GTSF-2. In addition to traditional growth-factors, GTSF-2 contains a blend of three sugars (glucose, galactose and fructose) at their physiological levels. For these studies, we isolated normal endothelial cells from human, bovine, and rat large blood vessels and microvessels. In addition, GTSF-2 was also tested as a replacement for high-glucose-containing medium for PC12 pheochromocytoma cells and for other, transformed cell lines. The cell growth characteristics were assessed with a novel cell viability and proliferation assay, which is based on the bioreduction of the fluorescent dye, Alamar Blue. After appropriate calibration, the Alamar Blue assay was found to be equivalent to established cell proliferation assays. Alamar Blue offers the advantage that cell proliferation can be measured in the same wells over an extended period of time. For some of the cell types (e.g., endothelial cells isolated from the bovine aorta, the rat adrenal medulla, or the transformed cells), proliferation in unmodified GTSF-2 was equivalent to that in the original culture media. For others cell types (e.g., human umbilical vein endothelial cells and PC12 cells), GTSF-2 proved to be a superior growth medium, when supplemented with simple additives, such as endothelial cells growth supplement (bFGF) or horse serum. Our results suggest that GTSF-2 is a versatile basal medium that will be useful for studying organ-specific in heterotypic coculture studies.
KW - Animals
KW - Cattle
KW - Cell Culture Techniques/methods
KW - Cell Division
KW - Cell Line, Transformed
KW - Cells, Cultured
KW - Coloring Agents
KW - Culture Media
KW - Endothelium, Vascular/cytology
KW - Humans
KW - Oxazines
KW - PC12 Cells
KW - Rats
KW - Xanthenes
UR - http://www.scopus.com/inward/record.url?scp=0030972127&partnerID=8YFLogxK
U2 - 10.1007/s11626-997-0004-7
DO - 10.1007/s11626-997-0004-7
M3 - Article
C2 - 9196892
SN - 1071-2690
VL - 33
SP - 344
EP - 351
JO - In Vitro Cellular and Developmental Biology - Animal
JF - In Vitro Cellular and Developmental Biology - Animal
IS - 5
ER -