Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

Conchi Estarás; Naiara Akizu; Alejandra García; Sergi Beltrán; Xavier de la Cruz; Marian A. Martínez-Balbás

doi:10.1242/dev.078345

Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

Conchi Estarás, Naiara Akizu, Alejandra García, Sergi Beltrán, Xavier de la Cruz, Marian A. Martínez-Balbás

Consejo Superior de Investigaciones Científicas

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Neural development requires crosstalk between signaling pathways and chromatin. In this study, we demonstrate that neurogenesis is promoted by an interplay between the TGFβ pathway and the H3K27me3 histone demethylase (HDM) JMJD3. Genome-wide analysis showed that JMJD3 is targeted to gene promoters by Smad3 in neural stem cells (NSCs) and is essential to activate TGFβ-responsive genes. In vivo experiments in chick spinal cord revealed that the generation of neurons promoted by Smad3 is dependent on JMJD3 HDM activity. Overall, these findings indicate that JMJD3 function is required for the TGFβ developmental program to proceed.

Original language	English
Pages (from-to)	2681-2691
Number of pages	11
Journal	Development (Cambridge)
Volume	139
Issue number	15
DOIs	https://doi.org/10.1242/dev.078345
State	Published - Aug 2012

Keywords

Animals
Chick Embryo
Developmental Biology/methods
Gene Expression Regulation
Genome-Wide Association Study
Green Fluorescent Proteins/metabolism
HEK293 Cells
Humans
Jumonji Domain-Containing Histone Demethylases/metabolism
Mice
Models, Biological
Neurons/metabolism
Oligonucleotide Array Sequence Analysis
Phosphorylation
Smad3 Protein/metabolism
Spinal Cord/embryology
Transforming Growth Factor beta/metabolism

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10.1242/dev.078345

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abstract = "Neural development requires crosstalk between signaling pathways and chromatin. In this study, we demonstrate that neurogenesis is promoted by an interplay between the TGFβ pathway and the H3K27me3 histone demethylase (HDM) JMJD3. Genome-wide analysis showed that JMJD3 is targeted to gene promoters by Smad3 in neural stem cells (NSCs) and is essential to activate TGFβ-responsive genes. In vivo experiments in chick spinal cord revealed that the generation of neurons promoted by Smad3 is dependent on JMJD3 HDM activity. Overall, these findings indicate that JMJD3 function is required for the TGFβ developmental program to proceed.",

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AU - Beltrán, Sergi

AU - de la Cruz, Xavier

AU - Martínez-Balbás, Marian A.

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Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.

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Keywords

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