TY - JOUR
T1 - Gene expression subtypes in patients with chronic fatigue syndrome/myalgic encephalomyelitis
AU - Kerr, Jonathan R.
AU - Petty, Robert
AU - Burke, Beverley
AU - Gough, John
AU - Fear, David
AU - Sinclair, Lindsey I.
AU - Mattey, Derek L.
AU - Richards, Selwyn C.M.
AU - Montgomery, Jane
AU - Baldwin, Don A.
AU - Kellam, Paul
AU - Harrison, Tim J.
AU - Griffin, George E.
AU - Main, Janice
AU - Enlander, Derek
AU - Nutt, David J.
AU - Holgate, Stephen T.
PY - 2008/4/15
Y1 - 2008/4/15
N2 - Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a multisystem disease, the pathogenesis of which remains undetermined. We set out to determine the precise abnormalities of gene expression in the blood of patients with CFS/ME. We analyzed gene expression in peripheral blood from 25 patients with CFS/ME diagnosed according to the Centers for Disease Control and Prevention diagnostic criteria and 50 healthy blood donors, using a microarray with a cutoff fold difference of expression of ≥2.5. Genes showing differential expression were further analyzed in 55 patients with CFS/ME and 75 healthy blood donors, using quantitative polymerase chain reaction. Differential expression was confirmed for 88 genes; 85 were upregulated, and 3 were downregulated. Highly represented functions were hematological disease and function, immunological disease and function, cancer, cell death, immune response, and infection. Clustering of quantitative polymerase chain reaction data from patients with CFS/ME revealed 7 subtypes with distinct differences in Medical Outcomes Survey Short Form-36 scores, clinical phenotypes, and severity.
AB - Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a multisystem disease, the pathogenesis of which remains undetermined. We set out to determine the precise abnormalities of gene expression in the blood of patients with CFS/ME. We analyzed gene expression in peripheral blood from 25 patients with CFS/ME diagnosed according to the Centers for Disease Control and Prevention diagnostic criteria and 50 healthy blood donors, using a microarray with a cutoff fold difference of expression of ≥2.5. Genes showing differential expression were further analyzed in 55 patients with CFS/ME and 75 healthy blood donors, using quantitative polymerase chain reaction. Differential expression was confirmed for 88 genes; 85 were upregulated, and 3 were downregulated. Highly represented functions were hematological disease and function, immunological disease and function, cancer, cell death, immune response, and infection. Clustering of quantitative polymerase chain reaction data from patients with CFS/ME revealed 7 subtypes with distinct differences in Medical Outcomes Survey Short Form-36 scores, clinical phenotypes, and severity.
KW - Adult
KW - Cluster Analysis
KW - Fatigue Syndrome, Chronic/blood
KW - Female
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Humans
KW - Male
KW - Multigene Family
KW - Oligonucleotide Array Sequence Analysis/methods
KW - Polymerase Chain Reaction
KW - Promoter Regions, Genetic
KW - RNA, Messenger/biosynthesis
KW - Transcription Factors/genetics
UR - http://www.scopus.com/inward/record.url?scp=42549113121&partnerID=8YFLogxK
U2 - 10.1086/533453
DO - 10.1086/533453
M3 - Article
C2 - 18462164
AN - SCOPUS:42549113121
SN - 0022-1899
VL - 197
SP - 1171
EP - 1184
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 8
ER -
