G protein complement of SV40-transformed ciliary epithelial cells

Harry Cooper; David R. Manning; Martin B. Wax

doi:10.3109/02713689008999615

G protein complement of SV40-transformed ciliary epithelial cells

Harry Cooper, David R. Manning, Martin B. Wax

University of Pennsylvania

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes G_sα and all three members of the G_iα family. Neither cell type contains G_oα or G_zα. Both cell lines contain β₃₅ and β₃₆. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.

Original language	English
Pages (from-to)	493-499
Number of pages	7
Journal	Current Eye Research
Volume	9
Issue number	5
DOIs	https://doi.org/10.3109/02713689008999615
State	Published - 1990

Keywords

Amino Acid Sequence
Animals
Blotting, Western
Cattle
Cell Line, Transformed
Cell Transformation, Viral
Ciliary Body/analysis
Electrophoresis, Polyacrylamide Gel
Epithelium/analysis
GTP-Binding Proteins/analysis
Humans
Immunoenzyme Techniques
Molecular Sequence Data
Simian virus 40

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10.3109/02713689008999615

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title = "G protein complement of SV40-transformed ciliary epithelial cells",

abstract = "Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes Gsα and all three members of the Giα family. Neither cell type contains Goα or Gzα. Both cell lines contain β35 and β36. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.",

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author = "Harry Cooper and Manning, {David R.} and Wax, {Martin B.}",

year = "1990",

doi = "10.3109/02713689008999615",

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pages = "493--499",

journal = "Current Eye Research",

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T1 - G protein complement of SV40-transformed ciliary epithelial cells

AU - Cooper, Harry

AU - Manning, David R.

AU - Wax, Martin B.

PY - 1990

Y1 - 1990

N2 - Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes Gsα and all three members of the Giα family. Neither cell type contains Goα or Gzα. Both cell lines contain β35 and β36. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.

AB - Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes Gsα and all three members of the Giα family. Neither cell type contains Goα or Gzα. Both cell lines contain β35 and β36. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.

KW - Amino Acid Sequence

KW - Animals

KW - Blotting, Western

KW - Cattle

KW - Cell Line, Transformed

KW - Cell Transformation, Viral

KW - Ciliary Body/analysis

KW - Electrophoresis, Polyacrylamide Gel

KW - Epithelium/analysis

KW - GTP-Binding Proteins/analysis

KW - Humans

KW - Immunoenzyme Techniques

KW - Molecular Sequence Data

KW - Simian virus 40

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DO - 10.3109/02713689008999615

M3 - Article

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SN - 0271-3683

VL - 9

SP - 493

EP - 499

JO - Current Eye Research

JF - Current Eye Research

IS - 5

ER -

G protein complement of SV40-transformed ciliary epithelial cells

Abstract

Keywords

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