Functional neuroendocrine tissue constructs generated in rotating wall vessel bioreactors

Peter I. Lelkes; Nasim Akhtar; Lydia Maltz; Efrat Lelkes; Brian R. Unsworth

Functional neuroendocrine tissue constructs generated in rotating wall vessel bioreactors

Peter I. Lelkes, Nasim Akhtar, Lydia Maltz, Efrat Lelkes, Brian R. Unsworth

Research output: Contribution to conference › Paper › peer-review

Abstract

The RWVs as a novel cell culture venue for the generation of macroscopic, differentiated 3-D tissue constructs was explored using PC12 pheochromocytoma cells as a cellular model. Biochemical, immunological, and molecular biological techniques, including cDNA arrays, were used to analyze signal transduction, catecholamine contents, and gene expression in 3-D tissue-like construct of PC12 cells grown for up to 30 d in RWVs. Vascularization of organoids implanted s.c. into Matrigel plugs in B56 mice was evaluated by fluorescence microscopy. It was observed that the unique cell culture environment in RWV bioreactors activated specific differentiative signaling pathways, facilitating engineering functional tissue constructs.

Original language	English
Pages	18
Number of pages	1
State	Published - 2002
Externally published	Yes
Event	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States Duration: Oct 13 2002 → Oct 16 2002

Conference

Conference	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products
Country/Territory	United States
City	Atlanta, GA
Period	10/13/02 → 10/16/02

Cite this

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title = "Functional neuroendocrine tissue constructs generated in rotating wall vessel bioreactors",

abstract = "The RWVs as a novel cell culture venue for the generation of macroscopic, differentiated 3-D tissue constructs was explored using PC12 pheochromocytoma cells as a cellular model. Biochemical, immunological, and molecular biological techniques, including cDNA arrays, were used to analyze signal transduction, catecholamine contents, and gene expression in 3-D tissue-like construct of PC12 cells grown for up to 30 d in RWVs. Vascularization of organoids implanted s.c. into Matrigel plugs in B56 mice was evaluated by fluorescence microscopy. It was observed that the unique cell culture environment in RWV bioreactors activated specific differentiative signaling pathways, facilitating engineering functional tissue constructs.",

author = "Lelkes, {Peter I.} and Nasim Akhtar and Lydia Maltz and Efrat Lelkes and Unsworth, {Brian R.}",

year = "2002",

language = "English",

pages = "18",

note = "Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products ; Conference date: 13-10-2002 Through 16-10-2002",

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TY - CONF

T1 - Functional neuroendocrine tissue constructs generated in rotating wall vessel bioreactors

AU - Lelkes, Peter I.

AU - Akhtar, Nasim

AU - Maltz, Lydia

AU - Lelkes, Efrat

AU - Unsworth, Brian R.

PY - 2002

Y1 - 2002

N2 - The RWVs as a novel cell culture venue for the generation of macroscopic, differentiated 3-D tissue constructs was explored using PC12 pheochromocytoma cells as a cellular model. Biochemical, immunological, and molecular biological techniques, including cDNA arrays, were used to analyze signal transduction, catecholamine contents, and gene expression in 3-D tissue-like construct of PC12 cells grown for up to 30 d in RWVs. Vascularization of organoids implanted s.c. into Matrigel plugs in B56 mice was evaluated by fluorescence microscopy. It was observed that the unique cell culture environment in RWV bioreactors activated specific differentiative signaling pathways, facilitating engineering functional tissue constructs.

AB - The RWVs as a novel cell culture venue for the generation of macroscopic, differentiated 3-D tissue constructs was explored using PC12 pheochromocytoma cells as a cellular model. Biochemical, immunological, and molecular biological techniques, including cDNA arrays, were used to analyze signal transduction, catecholamine contents, and gene expression in 3-D tissue-like construct of PC12 cells grown for up to 30 d in RWVs. Vascularization of organoids implanted s.c. into Matrigel plugs in B56 mice was evaluated by fluorescence microscopy. It was observed that the unique cell culture environment in RWV bioreactors activated specific differentiative signaling pathways, facilitating engineering functional tissue constructs.

UR - http://www.scopus.com/inward/record.url?scp=18644365733&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:18644365733

SP - 18

T2 - Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products

Y2 - 13 October 2002 through 16 October 2002

ER -

Functional neuroendocrine tissue constructs generated in rotating wall vessel bioreactors

Abstract

Conference

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