Fourier transform infrared spectroscopy of deuterated proteins

A. Marcano O.; Y. Markushin; N. Melikechi; D. Connolly

doi:10.1117/12.794878

Fourier transform infrared spectroscopy of deuterated proteins

A. Marcano O., Y. Markushin, N. Melikechi, D. Connolly

Delaware State University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

We report on Fourier transform spectra of deuterated proteins: Bovine Serum Albumin, Leptin, Insulin-like Growth Factor II, monoclonal antibody to ovarian cancer antigen CA125 and Osteopontin. The spectra exhibit changes in the relative amplitude and spectral width of certain peaks. New peaks not present in the non-deuterated sample are also observed. Ways for improving the deuteration of proteins by varying the temperature and dilution time are discussed. We propose the use of deuterated proteins to increase the sensitivity of immunoassays aimed for early diagnostic of diseases most notably cancer.

Original language	English
Title of host publication	Linear and Nonlinear Optics of Organic Materials VIII
DOIs	https://doi.org/10.1117/12.794878
State	Published - 2008
Event	Linear and Nonlinear Optics of Organic Materials VIII - San Diego, CA, United States Duration: Aug 12 2008 → Aug 14 2008

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7049
ISSN (Print)	0277-786X

Conference

Conference	Linear and Nonlinear Optics of Organic Materials VIII
Country/Territory	United States
City	San Diego, CA
Period	08/12/08 → 08/14/08

Keywords

Deuteration of proteins
Fourier transform spectroscopy
Immunoassay with deuterated proteins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.794878

Cite this

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title = "Fourier transform infrared spectroscopy of deuterated proteins",

abstract = "We report on Fourier transform spectra of deuterated proteins: Bovine Serum Albumin, Leptin, Insulin-like Growth Factor II, monoclonal antibody to ovarian cancer antigen CA125 and Osteopontin. The spectra exhibit changes in the relative amplitude and spectral width of certain peaks. New peaks not present in the non-deuterated sample are also observed. Ways for improving the deuteration of proteins by varying the temperature and dilution time are discussed. We propose the use of deuterated proteins to increase the sensitivity of immunoassays aimed for early diagnostic of diseases most notably cancer.",

keywords = "Deuteration of proteins, Fourier transform spectroscopy, Immunoassay with deuterated proteins",

author = "{Marcano O.}, A. and Y. Markushin and N. Melikechi and D. Connolly",

year = "2008",

doi = "10.1117/12.794878",

language = "English",

isbn = "9780819472694",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Linear and Nonlinear Optics of Organic Materials VIII",

note = "Linear and Nonlinear Optics of Organic Materials VIII ; Conference date: 12-08-2008 Through 14-08-2008",

}

Marcano O., A, Markushin, Y, Melikechi, N & Connolly, D 2008, Fourier transform infrared spectroscopy of deuterated proteins. in Linear and Nonlinear Optics of Organic Materials VIII., 70490Z, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7049, Linear and Nonlinear Optics of Organic Materials VIII, San Diego, CA, United States, 08/12/08. https://doi.org/10.1117/12.794878

Fourier transform infrared spectroscopy of deuterated proteins. / Marcano O., A.; Markushin, Y.; Melikechi, N. et al.
Linear and Nonlinear Optics of Organic Materials VIII. 2008. 70490Z (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7049).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Fourier transform infrared spectroscopy of deuterated proteins

AU - Marcano O., A.

AU - Markushin, Y.

AU - Melikechi, N.

AU - Connolly, D.

PY - 2008

Y1 - 2008

N2 - We report on Fourier transform spectra of deuterated proteins: Bovine Serum Albumin, Leptin, Insulin-like Growth Factor II, monoclonal antibody to ovarian cancer antigen CA125 and Osteopontin. The spectra exhibit changes in the relative amplitude and spectral width of certain peaks. New peaks not present in the non-deuterated sample are also observed. Ways for improving the deuteration of proteins by varying the temperature and dilution time are discussed. We propose the use of deuterated proteins to increase the sensitivity of immunoassays aimed for early diagnostic of diseases most notably cancer.

AB - We report on Fourier transform spectra of deuterated proteins: Bovine Serum Albumin, Leptin, Insulin-like Growth Factor II, monoclonal antibody to ovarian cancer antigen CA125 and Osteopontin. The spectra exhibit changes in the relative amplitude and spectral width of certain peaks. New peaks not present in the non-deuterated sample are also observed. Ways for improving the deuteration of proteins by varying the temperature and dilution time are discussed. We propose the use of deuterated proteins to increase the sensitivity of immunoassays aimed for early diagnostic of diseases most notably cancer.

KW - Deuteration of proteins

KW - Fourier transform spectroscopy

KW - Immunoassay with deuterated proteins

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DO - 10.1117/12.794878

M3 - Conference contribution

AN - SCOPUS:55549113573

SN - 9780819472694

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Linear and Nonlinear Optics of Organic Materials VIII

T2 - Linear and Nonlinear Optics of Organic Materials VIII

Y2 - 12 August 2008 through 14 August 2008

ER -

Fourier transform infrared spectroscopy of deuterated proteins

Abstract

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Conference

Keywords

UN SDGs

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