Expression of anaphase-promoting complex 5 in balloon angioplasty-injured rat carotid arteries and mitogen-stimulated human vascular smooth muscle cells

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We utilized differential display to identify differentially expressed mRNAs induced by balloon angioplasty injury. A recently described subunit of the anaphase-promoting complex, APC5, is not expressed in uninjured rat carotid arteries. Expression of APC5 mRNA is detectable as early as 1 day post-injury, reached maximal levels by 3-7 days, and declined by 14 days post-injury. APC5 is not expressed in serumstarved cultured human vascular smooth muscle cells (VSMC), but is strongly induced by mitogenic factors, and to a lesser extent by nonmitogenic cytokines. The kinetics of APC5 expression is associated with cell cycle progression, and corresponds with PCNA expression in PDGF-stimulated human VSMC. Interestingly, APC5 is expressed in most human tissues examined, regardless of their proliferative state. These data are the first description of the cytokine-inducible expression of APC5 and suggest that expression of this gene may represent an important event in the pathogenesis of vascular proliferative diseases.

Original languageEnglish
Pages (from-to)723-728
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number3
DOIs
StatePublished - 2001

Keywords

  • Amino Acid Sequence
  • Anaphase-Promoting Complex-Cyclosome
  • Angioplasty, Balloon/adverse effects
  • Animals
  • Apc5 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Carotid Artery Injuries
  • Carotid Artery, Common/metabolism
  • Cells, Cultured
  • Cytokines/pharmacology
  • DNA, Complementary/genetics
  • Fibroblast Growth Factors/pharmacology
  • Gene Expression
  • Humans
  • Ligases/genetics
  • Male
  • Mitogens/pharmacology
  • Molecular Sequence Data
  • Muscle, Smooth, Vascular/cytology
  • Platelet-Derived Growth Factor/pharmacology
  • Proliferating Cell Nuclear Antigen/metabolism
  • RNA, Messenger/genetics
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Nucleic Acid
  • Ubiquitin-Protein Ligase Complexes
  • Ubiquitin-Protein Ligases

Fingerprint

Dive into the research topics of 'Expression of anaphase-promoting complex 5 in balloon angioplasty-injured rat carotid arteries and mitogen-stimulated human vascular smooth muscle cells'. Together they form a unique fingerprint.

Cite this