@inbook{c355702048ee4abfa6c3ffb77cd4e56c,
title = "Experimental analysis of the mechanism of chromatin remodeling by rna polymerase ii",
abstract = "The vital process of transcription by RNA polymerase II (Pol II) occurs in chromatin environment in eukaryotic cells; in fact, moderately transcribed genes retain nucleosomal structure. Recent studies suggest that chromatin structure presents a strong barrier for transcribing Pol II in vitro, and that DNA-histone interactions are only partially and transiently disrupted during transcript elongation on moderately active genes. Furthermore, elongating Pol II complex is one of the major targets during gene regulation. Below, we describe a highly purified, defined experimental system that recapitulates many important properties of transcribed chromatin in vitro and allows detailed analysis of the underlying mechanisms.",
keywords = "DNA uncoiling, Histone octamer, Nucleosome, Structures, Transcript elongation",
author = "Gaykalova, {Daria A.} and Kulaeva, {Olga I.} and Pestov, {Nikolai A.} and Hsieh, {Fu Kai} and Studitsky, {Vasily M.}",
year = "2012",
doi = "10.1016/B978-0-12-391940-3.00013-5",
language = "English",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "293--314",
booktitle = "Methods in Enzymology",
address = "United States",
}