Endothelial cell seeding with rotation of a ventricular blood sac

D. M. Wankowski, M. M. Samet, V. V. Nikolaychik, P. I. Lelkes

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Successful establishment of a durable endothelial cell (EC) monolayer inside a ventricular blood sac requires homogeneous coverage of the entire luminal surface with attached cells. For this purpose, a new device was developed that slowly rotates a fully assembled cardiac prosthesis with three degrees of freedom. We seeded ECs derived from human adipose tissue at a density of ≃ 3.5 x 104 cells/cm2 onto the surfaces of polyurethane-made blood sacs and 'ersatz' bladders (consisting of T-25 tissue culture flasks). The kinetics of cell attachment, spreading, and proliferation were determined using video microscopy combined with image analysis and cell viability assays. After 60 min of seeding at 5-10 rotations/hr, the plating efficiency inside the blood sacs was 35.7 ± 11%, with cell viability remaining ≃ 90 ± 5%. After 3 hr, when the plating efficiency reached a plateau (≃ 70%), the rotation was stopped and the ECs were allowed to spread and proliferate under static conditions. Within 48 hr, the entire luminal surface was evenly covered by a confluent EC monolayer. Our long-term studies show that with a proper feeding schedule, such an EC monolayer can be maintained intact in vitro for more than 2 weeks.

Original languageEnglish
Pages (from-to)M319-M324
JournalASAIO Journal
Volume40
Issue number3
DOIs
StatePublished - 1994

Keywords

  • Adipose Tissue/blood supply
  • Biomedical Engineering
  • Blood
  • Cell Adhesion
  • Cell Division
  • Cells, Cultured
  • Endothelium, Vascular/cytology
  • Evaluation Studies as Topic
  • Heart-Assist Devices
  • Humans
  • In Vitro Techniques
  • Microscopy, Electron, Scanning
  • Rotation

Fingerprint

Dive into the research topics of 'Endothelial cell seeding with rotation of a ventricular blood sac'. Together they form a unique fingerprint.

Cite this