TY - GEN
T1 - Electrospun blends of natural and synthetic polymers as scaffolds for tissue engineering
AU - Li, Mengyan
AU - Mondrinos, Mark J.
AU - Chen, Xuesi
AU - Lelkes, Peter I.
PY - 2005
Y1 - 2005
N2 - Engineering functional three-dimensional (3-D) tissue constructs for the replacement and/or repair of damaged native tissues using cells and scaffolds is one of the ultimate goals of tissue engineering. In this study, non-woven fibrous scaffolds were electrospun from the synthetic biodegradable polymer poly(lactic-co-glycolic acid) (PLGA) and natural proteins, gelatin (denatured collagen) and elastin. In the absence of cross-linking agent, the average PGE fiber diameter increased from 347 ± 103 nm to 999 ± 123 nm upon wetting as measured by scanning electron microscopy. Rat bone marrow stromal cells (rBMSC) were used paradigmatically to study the 3-D cell culture properties of PGE scaffolds. Consistent with the observed properties of the individual fibers, PGE scaffolds initially swelled in aqueous culture medium, however rBMSC seeded PGE scaffolds contracted to < 50% of original size. Time course histological analysis demonstrated uniform seeding of rBMSC into PGE scaffolds and complete cell penetration into the fibrous architecture over 4 weeks of in vitro culture.
AB - Engineering functional three-dimensional (3-D) tissue constructs for the replacement and/or repair of damaged native tissues using cells and scaffolds is one of the ultimate goals of tissue engineering. In this study, non-woven fibrous scaffolds were electrospun from the synthetic biodegradable polymer poly(lactic-co-glycolic acid) (PLGA) and natural proteins, gelatin (denatured collagen) and elastin. In the absence of cross-linking agent, the average PGE fiber diameter increased from 347 ± 103 nm to 999 ± 123 nm upon wetting as measured by scanning electron microscopy. Rat bone marrow stromal cells (rBMSC) were used paradigmatically to study the 3-D cell culture properties of PGE scaffolds. Consistent with the observed properties of the individual fibers, PGE scaffolds initially swelled in aqueous culture medium, however rBMSC seeded PGE scaffolds contracted to < 50% of original size. Time course histological analysis demonstrated uniform seeding of rBMSC into PGE scaffolds and complete cell penetration into the fibrous architecture over 4 weeks of in vitro culture.
KW - Elastin, tissue engineering
KW - Electrospinning
KW - Nanofibrous scaffold
KW - PLGA, gelatin
UR - http://www.scopus.com/inward/record.url?scp=33846911594&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:000238998405070&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1109/IEMBS.2005.1615822
DO - 10.1109/IEMBS.2005.1615822
M3 - Conference contribution
C2 - 17281592
SN - 0780387406
SN - 9780780387409
VL - 2005
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 5858
EP - 5861
BT - Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
T2 - 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Y2 - 1 September 2005 through 4 September 2005
ER -