Effect of Single-Strand DNA Breaks on Transcription of Nucleosomes

N. S. Gerasimova; M. S. Akhtar; V. M. Studitskii

doi:10.3103/S0096392522040022

Effect of Single-Strand DNA Breaks on Transcription of Nucleosomes

N. S. Gerasimova, M. S. Akhtar, V. M. Studitskii

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Abstract

Abstract–: Previous studies have revealed the inhibitory effect of single-strand breaks in a non-template DNA strand (NT-SSB) on RNA polymerase (RNAP) transcription through the nucleosome. The observed effect was explained within the model of chromatin transcription mechanism with the formation of intranucleosomal DNA loops (i-loops), intermediates, in which the enzyme is locked in the DNA loop on the histone octamer. According to the model, NT-SSBs reduce the tension in the DNA structure caused by transcription, thus stabilizing DNA-histone interactions and inhibiting RNAP. In this work, the boundaries of such i-loops have been determined. It has been found that the formation of contacts between DNA and histones behind RNAP occurs at a distance of more than 17 base pairs from the active center of the enzyme.

Original language	English
Pages (from-to)	216-222
Number of pages	7
Journal	Moscow University Biological Sciences Bulletin
Volume	77
Issue number	4
DOIs	https://doi.org/10.3103/S0096392522040022
State	Published - Dec 2022

Keywords

DNA repair
RNAP2
chromatin
i-loop
nucleosome
single-stranded breaks
transcription

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10.3103/S0096392522040022

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title = "Effect of Single-Strand DNA Breaks on Transcription of Nucleosomes",

abstract = "Abstract–: Previous studies have revealed the inhibitory effect of single-strand breaks in a non-template DNA strand (NT-SSB) on RNA polymerase (RNAP) transcription through the nucleosome. The observed effect was explained within the model of chromatin transcription mechanism with the formation of intranucleosomal DNA loops (i-loops), intermediates, in which the enzyme is locked in the DNA loop on the histone octamer. According to the model, NT-SSBs reduce the tension in the DNA structure caused by transcription, thus stabilizing DNA-histone interactions and inhibiting RNAP. In this work, the boundaries of such i-loops have been determined. It has been found that the formation of contacts between DNA and histones behind RNAP occurs at a distance of more than 17 base pairs from the active center of the enzyme.",

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T1 - Effect of Single-Strand DNA Breaks on Transcription of Nucleosomes

AU - Gerasimova, N. S.

AU - Akhtar, M. S.

AU - Studitskii, V. M.

PY - 2022/12

Y1 - 2022/12

N2 - Abstract–: Previous studies have revealed the inhibitory effect of single-strand breaks in a non-template DNA strand (NT-SSB) on RNA polymerase (RNAP) transcription through the nucleosome. The observed effect was explained within the model of chromatin transcription mechanism with the formation of intranucleosomal DNA loops (i-loops), intermediates, in which the enzyme is locked in the DNA loop on the histone octamer. According to the model, NT-SSBs reduce the tension in the DNA structure caused by transcription, thus stabilizing DNA-histone interactions and inhibiting RNAP. In this work, the boundaries of such i-loops have been determined. It has been found that the formation of contacts between DNA and histones behind RNAP occurs at a distance of more than 17 base pairs from the active center of the enzyme.

AB - Abstract–: Previous studies have revealed the inhibitory effect of single-strand breaks in a non-template DNA strand (NT-SSB) on RNA polymerase (RNAP) transcription through the nucleosome. The observed effect was explained within the model of chromatin transcription mechanism with the formation of intranucleosomal DNA loops (i-loops), intermediates, in which the enzyme is locked in the DNA loop on the histone octamer. According to the model, NT-SSBs reduce the tension in the DNA structure caused by transcription, thus stabilizing DNA-histone interactions and inhibiting RNAP. In this work, the boundaries of such i-loops have been determined. It has been found that the formation of contacts between DNA and histones behind RNAP occurs at a distance of more than 17 base pairs from the active center of the enzyme.

KW - DNA repair

KW - RNAP2

KW - chromatin

KW - i-loop

KW - nucleosome

KW - single-stranded breaks

KW - transcription

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SP - 216

EP - 222

JO - Moscow University Biological Sciences Bulletin

JF - Moscow University Biological Sciences Bulletin

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ER -

Effect of Single-Strand DNA Breaks on Transcription of Nucleosomes

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Keywords

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