DNA supercoiling allows enhancer action over a large distance

Ye Liu; Vladimir Bondarenko; Alexander Ninfa; Vasily M. Studitsky

doi:10.1073/pnas.261477898

DNA supercoiling allows enhancer action over a large distance

Ye Liu, Vladimir Bondarenko, Alexander Ninfa, Vasily M. Studitsky

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Enhancers are regulatory DNA elements that can activate their genomic targets over a large distance. The mechanism of enhancer action over large distance is unknown. Activation of the glnAp2 promoter by NtrC-dependent enhancer in Escherichia coli was analyzed by using a purified system supporting multiple-round transcription in vitro. The data suggest that DNA supercoiling is an essential requirement for enhancer action over a large distance (2,500 bp) but not over a short distance (110 bp). DNA supercoiling facilitates functional enhancer-promoter communication over a large distance, probably by bringing the enhancer and promoter into close proximity.

Original language	English
Pages (from-to)	14883-14888
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	98
Issue number	26
DOIs	https://doi.org/10.1073/pnas.261477898
State	Published - Dec 18 2001

Keywords

Base Sequence
DNA Primers
DNA, Superhelical/physiology
Enhancer Elements, Genetic
Escherichia coli/genetics
Gene Expression Regulation, Bacterial
Promoter Regions, Genetic
Transcription, Genetic

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10.1073/pnas.261477898

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AU - Liu, Ye

AU - Bondarenko, Vladimir

AU - Ninfa, Alexander

AU - Studitsky, Vasily M.

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Y1 - 2001/12/18

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AB - Enhancers are regulatory DNA elements that can activate their genomic targets over a large distance. The mechanism of enhancer action over large distance is unknown. Activation of the glnAp2 promoter by NtrC-dependent enhancer in Escherichia coli was analyzed by using a purified system supporting multiple-round transcription in vitro. The data suggest that DNA supercoiling is an essential requirement for enhancer action over a large distance (2,500 bp) but not over a short distance (110 bp). DNA supercoiling facilitates functional enhancer-promoter communication over a large distance, probably by bringing the enhancer and promoter into close proximity.

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KW - DNA Primers

KW - DNA, Superhelical/physiology

KW - Enhancer Elements, Genetic

KW - Escherichia coli/genetics

KW - Gene Expression Regulation, Bacterial

KW - Promoter Regions, Genetic

KW - Transcription, Genetic

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JO - Proceedings of the National Academy of Sciences of the United States of America

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DNA supercoiling allows enhancer action over a large distance

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