Disruption of Telomerase RNA Maturation Kinetics Precipitates Disease

Mutations in RNA-processing enzymes are increasingly linked to human disease. Telomerase RNA and related noncoding RNAs require 3′ end-processing steps, including oligoadenylation. Germline mutations in poly(A)ribonuclease (PARN) cause accumulation of extended human telomerase RNA (hTR) species and precipitate dyskeratosis congenita and pulmonary fibrosis. Here, we develop nascent RNAend-seq to measure processing rates of RNA precursors. We find that mature hTR derives from extended precursors but that in PARN-mutant cells hTR maturation kinetically stalls and unprocessed precursors are degraded. Loss of poly(A)polymerase PAPD5 in PARN-mutant cells accelerates hTR maturation and restores hTR processing, indicating that oligoadenylation and deadenylation set rates of hTR maturation. The H/ACA domain mediates hTR maturation by precisely defining the 3′ end, recruiting poly(A)polymerase activity, and conferring sensitivity to PARN regulation. These data reveal a feedforward circuit in which post-transcriptional oligoadenylation controls RNA maturation kinetics. Similar alterations in RNA processing rates may contribute to mechanisms of RNA-based human disease. Mutations in poly(A)ribonuclease (PARN) cause accumulation of telomerase RNA component (hTR) precursors and underlie a subset of dyskeratosis congenita. Using nascent RNAend-Seq to measure maturation rates of ncRNA precursors, Roake et al. show that posttranscriptional oligoadenylation by PAPD5 and deadenylation by PARN set hTR maturation rate and determine telomerase levels.