TY - JOUR
T1 - Direct fluorometric assay of catecholamine secretion from isolated bovine adrenal chromaffin cells
AU - Lelkes, Peter I.
AU - Friedman, Jonathan E.
AU - Rosenheck, Kurt
PY - 1985/5
Y1 - 1985/5
N2 - The intrinsic fluorescence of catecholamines can be exploited for a simple and speedy fluorescence assay of secretion from bovine adrenal chromaffin cells. Catecholamines are separated from concomitantly secreted proteins by centrifugation in 0.4 M perchloric acid, and their fluorescence is measured directly in a conventional spectrofluorometer without chemical derivatization. Catecholamine release, assayed by this technique, is virtually identical to data obtained in parallel by the classical trihydroxyindole method. Intrinsic catecholamine fluorescence does not differentiate between epinephrine and norepinephrine. Interferences from small molecular weight compounds not precipitated in perchloric acid are rare, and can be accounted for using appropriate calibration curves.
AB - The intrinsic fluorescence of catecholamines can be exploited for a simple and speedy fluorescence assay of secretion from bovine adrenal chromaffin cells. Catecholamines are separated from concomitantly secreted proteins by centrifugation in 0.4 M perchloric acid, and their fluorescence is measured directly in a conventional spectrofluorometer without chemical derivatization. Catecholamine release, assayed by this technique, is virtually identical to data obtained in parallel by the classical trihydroxyindole method. Intrinsic catecholamine fluorescence does not differentiate between epinephrine and norepinephrine. Interferences from small molecular weight compounds not precipitated in perchloric acid are rare, and can be accounted for using appropriate calibration curves.
KW - Adrenal Medulla/metabolism
KW - Animals
KW - Catecholamines/analysis
KW - Cattle
KW - Cells, Cultured
KW - Spectrometry, Fluorescence
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UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1985AKB0200009&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1016/0165-0270(85)90073-1
DO - 10.1016/0165-0270(85)90073-1
M3 - Article
C2 - 4010334
SN - 0165-0270
VL - 13
SP - 249
EP - 255
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 3-4
ER -