TY - JOUR
T1 - Direct evidence for the deoxyribonuclease activity of the plant ribosome inactivating protein gelonin
AU - Nicolas, Emmanuelle
AU - Beggs, Joseph M.
AU - Haltiwanger, Brett M.
AU - Taraschi, Theodore F.
PY - 1997/4/7
Y1 - 1997/4/7
N2 - Several plant ribotoxins, including gelonin, were reported to have additional weak nuclease activities on supercoiled DNA. The potential contribution of this activity to their cytotoxicity has not been given serious consideration due to concerns about contaminating nucleases in the protein preparations. We now report the degradation of single-stranded DNA by preparations of native plant gelonin and recombinant gelonin produced in E. coli. The DNase activity of both preparations is similarly modulated by zinc. An SDS-PAGE DNase assay identifies gelonin as the polypeptide responsible for deoxyribonuclease activity.
AB - Several plant ribotoxins, including gelonin, were reported to have additional weak nuclease activities on supercoiled DNA. The potential contribution of this activity to their cytotoxicity has not been given serious consideration due to concerns about contaminating nucleases in the protein preparations. We now report the degradation of single-stranded DNA by preparations of native plant gelonin and recombinant gelonin produced in E. coli. The DNase activity of both preparations is similarly modulated by zinc. An SDS-PAGE DNase assay identifies gelonin as the polypeptide responsible for deoxyribonuclease activity.
KW - DNA, Single-Stranded/drug effects
KW - Deoxyribonucleases/metabolism
KW - Plant Proteins/metabolism
KW - Plants/metabolism
KW - Recombinant Proteins/metabolism
KW - Ribosome Inactivating Proteins, Type 1
KW - Ribosomes/drug effects
UR - http://www.scopus.com/inward/record.url?scp=0030992505&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(97)00267-6
DO - 10.1016/S0014-5793(97)00267-6
M3 - Article
C2 - 9109409
AN - SCOPUS:0030992505
SN - 0014-5793
VL - 406
SP - 162
EP - 164
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -