Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?

Eric M. Troop; Amir Rezvan; Fred D. Allen; Brian R. Unsworth; Peter I. Lelkes

Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?

Eric M. Troop, Amir Rezvan, Fred D. Allen, Brian R. Unsworth, Peter I. Lelkes

Research output: Contribution to conference › Paper › peer-review

Abstract

The potential role of simulated/modeled microgravity conditions in differential gene expression in cells cultured in rotating wall vessel (RWV) bioreactors was investigated. The patterns of differential gene expression in rat adrenal medullary endothalial cells (RAMEC) cultured for 24 hours, either in RWV bioreactors in static monolayers, static suspension cultures, or as monolayers in a parallel plate flow chamber exposed to fluid shear stress, were compared. It was observed that compared to static monolayer cultures, the expression of almost 19% genes was affected by one or more of the alternate culture conditions. The results suggest that simulated microgravity conditions are important for the alterations in gene expression in RWV bioreactors.

Original language	English
Pages	132
Number of pages	1
State	Published - 2002
Externally published	Yes
Event	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States Duration: Oct 13 2002 → Oct 16 2002

Conference

Conference	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products
Country/Territory	United States
City	Atlanta, GA
Period	10/13/02 → 10/16/02

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Troop, E. M., Rezvan, A., Allen, F. D., Unsworth, B. R., & Lelkes, P. I. (2002). Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?. 132. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Troop, Eric M. ; Rezvan, Amir ; Allen, Fred D. et al. / Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress : Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.1 p.

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title = "Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of {"}simulated/modeled microgravity{"} conditions in gene activation?",

abstract = "The potential role of simulated/modeled microgravity conditions in differential gene expression in cells cultured in rotating wall vessel (RWV) bioreactors was investigated. The patterns of differential gene expression in rat adrenal medullary endothalial cells (RAMEC) cultured for 24 hours, either in RWV bioreactors in static monolayers, static suspension cultures, or as monolayers in a parallel plate flow chamber exposed to fluid shear stress, were compared. It was observed that compared to static monolayer cultures, the expression of almost 19% genes was affected by one or more of the alternate culture conditions. The results suggest that simulated microgravity conditions are important for the alterations in gene expression in RWV bioreactors.",

author = "Troop, {Eric M.} and Amir Rezvan and Allen, {Fred D.} and Unsworth, {Brian R.} and Lelkes, {Peter I.}",

year = "2002",

language = "English",

pages = "132",

note = "Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products ; Conference date: 13-10-2002 Through 16-10-2002",

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Troop, EM, Rezvan, A, Allen, FD, Unsworth, BR & Lelkes, PI 2002, 'Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?', Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States, 10/13/02 - 10/16/02 pp. 132.

Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation? / Troop, Eric M.; Rezvan, Amir; Allen, Fred D. et al.
2002. 132 Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress

T2 - Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products

AU - Troop, Eric M.

AU - Rezvan, Amir

AU - Allen, Fred D.

AU - Unsworth, Brian R.

AU - Lelkes, Peter I.

PY - 2002

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AB - The potential role of simulated/modeled microgravity conditions in differential gene expression in cells cultured in rotating wall vessel (RWV) bioreactors was investigated. The patterns of differential gene expression in rat adrenal medullary endothalial cells (RAMEC) cultured for 24 hours, either in RWV bioreactors in static monolayers, static suspension cultures, or as monolayers in a parallel plate flow chamber exposed to fluid shear stress, were compared. It was observed that compared to static monolayer cultures, the expression of almost 19% genes was affected by one or more of the alternate culture conditions. The results suggest that simulated microgravity conditions are important for the alterations in gene expression in RWV bioreactors.

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M3 - Paper

AN - SCOPUS:18644377733

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Y2 - 13 October 2002 through 16 October 2002

ER -

Troop EM, Rezvan A, Allen FD, Unsworth BR, Lelkes PI. Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?. 2002. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Differential gene expression in endothelial cells cultured in rotating wall vessel bioreactors and exposed to low laminar shear stress: Evidence for a potential role of "simulated/modeled microgravity" conditions in gene activation?

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