Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.

Vladimir Khazak; Erica A. Golemis; Lutz Weber

doi:10.1007/978-1-59259-948-6_18

Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.

Vladimir Khazak, Erica A. Golemis, Lutz Weber

NexusPharma, Inc.

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.

Original language	English
Pages (from-to)	253-271
Number of pages	19
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	310
DOIs	https://doi.org/10.1007/978-1-59259-948-6_18
State	Published - 2005
Externally published	Yes

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10.1007/978-1-59259-948-6_18

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title = "Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.",

abstract = "A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.",

author = "Vladimir Khazak and Golemis, {Erica A.} and Lutz Weber",

year = "2005",

doi = "10.1007/978-1-59259-948-6_18",

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AU - Golemis, Erica A.

AU - Weber, Lutz

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Y1 - 2005

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AB - A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.

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U2 - 10.1007/978-1-59259-948-6_18

DO - 10.1007/978-1-59259-948-6_18

M3 - Article

SN - 1064-3745

VL - 310

SP - 253

EP - 271

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.

Abstract

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