TY - JOUR
T1 - Detection of Epstein-Barr virus in T-cell prolymphocytic leukemia cells in vitro
AU - Lan, Ke
AU - Murakami, Masanao
AU - Choudhuri, Tathagata
AU - Tsai, Donald E.
AU - Schuster, Stephen J.
AU - Wasik, Mariusz A.
AU - Robertson, Erle S.
PY - 2008/11
Y1 - 2008/11
N2 - Background: Epstein-Barr virus (EBV) is closely associated with the development of a number of tumors. During latent infection, EBV continuously expresses a number of viral genes which are essential for cell transformation and maintenance of the malignant phenotype of EBV-related tumors. There has been no previous link between EBV and T-cell prolymphocytic leukemia (T-PLL), a distinctive form of leukemia derived from T-cells at an intermediate stage of differentiation between a cortical thymocyte and a mature peripheral blood T-cell. Objective: To determine if EBV was present in the T-PLL cells collected. Study design: T-PLL cells were isolated from the peripheral blood of a patient diagnosed with T-PLL and continuously cultured for about 1 year. The existence of EBV in these cells was detected using multiple strategies including PCR, Western blotting, immunofluorescent assay and flow cytometry analysis. Results: The EBV genome was present in these T-PLL cells by PCR analysis across multiple sites in the viral genome. In addition, these T-PLL cells expressed a number of EBV latent antigens. The EBV oncoproteins LMP1, EBNA1 and EBNA3C were expressed in the majority of the infected cells. Conclusion: This report suggests a potential link between EBV infection and T-PLL and provides new information about the potential contribution of EBV in the initiation or maintenance of T-PLL.
AB - Background: Epstein-Barr virus (EBV) is closely associated with the development of a number of tumors. During latent infection, EBV continuously expresses a number of viral genes which are essential for cell transformation and maintenance of the malignant phenotype of EBV-related tumors. There has been no previous link between EBV and T-cell prolymphocytic leukemia (T-PLL), a distinctive form of leukemia derived from T-cells at an intermediate stage of differentiation between a cortical thymocyte and a mature peripheral blood T-cell. Objective: To determine if EBV was present in the T-PLL cells collected. Study design: T-PLL cells were isolated from the peripheral blood of a patient diagnosed with T-PLL and continuously cultured for about 1 year. The existence of EBV in these cells was detected using multiple strategies including PCR, Western blotting, immunofluorescent assay and flow cytometry analysis. Results: The EBV genome was present in these T-PLL cells by PCR analysis across multiple sites in the viral genome. In addition, these T-PLL cells expressed a number of EBV latent antigens. The EBV oncoproteins LMP1, EBNA1 and EBNA3C were expressed in the majority of the infected cells. Conclusion: This report suggests a potential link between EBV infection and T-PLL and provides new information about the potential contribution of EBV in the initiation or maintenance of T-PLL.
KW - Epstein-Barr virus
KW - Infection
KW - T-cell prolymphocytic leukemia
UR - http://www.scopus.com/inward/record.url?scp=53649106005&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2008.07.018
DO - 10.1016/j.jcv.2008.07.018
M3 - Article
C2 - 18790666
AN - SCOPUS:53649106005
SN - 1386-6532
VL - 43
SP - 260
EP - 265
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
IS - 3
ER -