Cytofluorometric quantification of cell death elicited by NLR proteins

Valentina Sica; Gwenola Manic; Guido Kroemer; Ilio Vitale; Lorenzo Galluzzi

doi:10.1007/978-1-4939-3566-6_17

Cytofluorometric quantification of cell death elicited by NLR proteins

Valentina Sica
, Gwenola Manic
, Guido Kroemer
, Ilio Vitale
, Lorenzo Galluzzi

Institut national de la santé et de la recherche médicale
Centre de Recherche des Cordeliers
Université Paris-Saclay
Sorbonne Université
IRCCS Istituti fisioterapici ospitalieri - Istituto Regina Elena
Université Paris Cité
Assistance publique – Hôpitaux de Paris
Metabolomics and Cell Biology Platforms
University of Rome Tor Vergata

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	231-245
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-4939-3566-6_17
State	Published - 2016
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1417
ISSN (Print)	1064-3745

Keywords

Annexin A5
Apoptosis
Caspase
Mitochondria
Necrosis

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10.1007/978-1-4939-3566-6_17

Cite this

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title = "Cytofluorometric quantification of cell death elicited by NLR proteins",

abstract = "Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.",

keywords = "Annexin A5, Apoptosis, Caspase, Mitochondria, Necrosis",

author = "Valentina Sica and Gwenola Manic and Guido Kroemer and Ilio Vitale and Lorenzo Galluzzi",

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T1 - Cytofluorometric quantification of cell death elicited by NLR proteins

AU - Sica, Valentina

AU - Manic, Gwenola

AU - Kroemer, Guido

AU - Vitale, Ilio

AU - Galluzzi, Lorenzo

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.

AB - Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.

KW - Annexin A5

KW - Apoptosis

KW - Caspase

KW - Mitochondria

KW - Necrosis

UR - https://www.scopus.com/pages/publications/84990190542

U2 - 10.1007/978-1-4939-3566-6_17

DO - 10.1007/978-1-4939-3566-6_17

M3 - Chapter

C2 - 27221495

AN - SCOPUS:84990190542

T3 - Methods in Molecular Biology

SP - 231

EP - 245

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Cytofluorometric quantification of cell death elicited by NLR proteins

Abstract

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Keywords

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