Cyclin/cdk2 Complexes in the Nucleus of HeLa Cells

Montserrat Jaumot, Xavier Graña, Antonio Giordano, Prem Veer Reddy, Neus Agell, Oriol Bachs

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Two different fractions of cdk2 and cdc2 have been found in the nucleus of HeLa cells. One, which can be extracted by nuclease treatment, possibly associated with DNA- or RNA-containing structures and another one, which is bound to the nuclear matrix. Nuclear cdk2 forms high molecular weight complexes which migrate at the same position as DNA polymerase α and proliferating cell nuclear antigen in sucrose gradient centrifugation experiments. These results suggest that nuclear cdk2 complexes could be associated with the replication factories. Immunoprecipitation experiments reveal that nuclear cdk2 complexes display histone H1-kinase activity and phosphorylate a protein of 18 kDa which is present in these complexes.

Original languageEnglish
Pages (from-to)1527-1534
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number3
DOIs
StatePublished - 1994

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