Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

Richard H.G. Baxter; Brandon Luke Seagle; Nina Ponomarenko; James R. Norris

doi:10.1107/S0907444905005809

Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

Richard H.G. Baxter
, Brandon Luke Seagle
, Nina Ponomarenko
, James R. Norris

Cancer Signaling and Microenvironment (CSM)

University of Chicago
University of Texas Southwestern Medical Center

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 A resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q(B)-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q(B) in the proximal position in both the neutral and charge-separated states.

Original language	English
Pages (from-to)	605-612
Number of pages	8
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	61
Issue number	5
DOIs	https://doi.org/10.1107/S0907444905005809
State	Published - May 2005

Keywords

Binding Sites
Cryoprotective Agents/chemistry
Crystallization
Darkness
Freezing
Herbicides/pharmacology
Light
Models, Molecular
Photosynthesis/physiology
Proteobacteria/metabolism
Quinones/chemistry
Ubiquinone/metabolism
Water/chemistry

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10.1107/S0907444905005809

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title = "Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark",

abstract = "The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 A resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q(B)-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q(B) in the proximal position in both the neutral and charge-separated states.",

keywords = "Binding Sites, Cryoprotective Agents/chemistry, Crystallization, Darkness, Freezing, Herbicides/pharmacology, Light, Models, Molecular, Photosynthesis/physiology, Proteobacteria/metabolism, Quinones/chemistry, Ubiquinone/metabolism, Water/chemistry",

author = "Baxter, \{Richard H.G.\} and Seagle, \{Brandon Luke\} and Nina Ponomarenko and Norris, \{James R.\}",

year = "2005",

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language = "English",

volume = "61",

pages = "605--612",

journal = "Acta Crystallographica Section D: Biological Crystallography",

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publisher = "International Union of Crystallography",

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T1 - Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

AU - Baxter, Richard H.G.

AU - Seagle, Brandon Luke

AU - Ponomarenko, Nina

AU - Norris, James R.

PY - 2005/5

Y1 - 2005/5

N2 - The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 A resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q(B)-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q(B) in the proximal position in both the neutral and charge-separated states.

AB - The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 A resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q(B)-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q(B) in the proximal position in both the neutral and charge-separated states.

KW - Binding Sites

KW - Cryoprotective Agents/chemistry

KW - Crystallization

KW - Darkness

KW - Freezing

KW - Herbicides/pharmacology

KW - Light

KW - Models, Molecular

KW - Photosynthesis/physiology

KW - Proteobacteria/metabolism

KW - Quinones/chemistry

KW - Ubiquinone/metabolism

KW - Water/chemistry

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U2 - 10.1107/S0907444905005809

DO - 10.1107/S0907444905005809

M3 - Article

C2 - 15858271

AN - SCOPUS:22844445485

SN - 0907-4449

VL - 61

SP - 605

EP - 612

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 5

ER -

Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

Abstract

Keywords

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