Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

Richard H.G. Baxter; Brandon Luke Seagle; Nina Ponomarenko; James R. Norris

doi:10.1107/S0907444905005809

Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

Richard H.G. Baxter, Brandon Luke Seagle, Nina Ponomarenko, James R. Norris

Cancer Signaling and Microenvironment (CSM)

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 A resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q(B)-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q(B) in the proximal position in both the neutral and charge-separated states.

Original language	English
Pages (from-to)	605-612
Number of pages	8
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	61
Issue number	5
DOIs	https://doi.org/10.1107/S0907444905005809
State	Published - May 2005

Keywords

Binding Sites
Cryoprotective Agents/chemistry
Crystallization
Darkness
Freezing
Herbicides/pharmacology
Light
Models, Molecular
Photosynthesis/physiology
Proteobacteria/metabolism
Quinones/chemistry
Ubiquinone/metabolism
Water/chemistry

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10.1107/S0907444905005809

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title = "Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark",

abstract = "The structure of the Blastochloris viridis photosynthetic reaction center has been determined at 100 K by flash-freezing crystals. A data set to 2.2 A resolution provides a well determined model of the wild-type protein. Of particular interest are the position, occupancy and heterogeneity of the Q(B)-binding site. Data were also collected from a crystal frozen immediately after illumination. The data support predominant binding of Q(B) in the proximal position in both the neutral and charge-separated states.",

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AU - Seagle, Brandon Luke

AU - Ponomarenko, Nina

AU - Norris, James R.

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KW - Crystallization

KW - Darkness

KW - Freezing

KW - Herbicides/pharmacology

KW - Light

KW - Models, Molecular

KW - Photosynthesis/physiology

KW - Proteobacteria/metabolism

KW - Quinones/chemistry

KW - Ubiquinone/metabolism

KW - Water/chemistry

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SN - 0907-4449

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JF - Acta Crystallographica Section D: Biological Crystallography

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ER -

Cryogenic structure of the photosynthetic reaction center of Blastochloris viridis in the light and dark

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Keywords

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