Conjugal transfer and stability of the plasmids determining exopolysaccharide production in Lactococcus lactis strains

Ela Koçer; Çaǧla Tükel; Mustafa Akçelik

Conjugal transfer and stability of the plasmids determining exopolysaccharide production in Lactococcus lactis strains

Ela Koçer, Çaǧla Tükel, Mustafa Akçelik

Ankara University

Research output: Contribution to journal › Article › peer-review

Abstract

A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps⁺) and lactose fermentation (Lac⁺) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac⁺/Eps⁺ plasmids were determined as 1.0 × 10^-2 - 2.8 × 10^-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg⁺) wild type strains and Agg⁺ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac⁺/Eps⁺ plasmids occurred as 1.5 × 10^-3 - 4.5 × 10^-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg^-) recipient strain L. lactis subsp. lactis ELL42-6.

Original language	English
Pages (from-to)	481-487
Number of pages	7
Journal	Turkish Journal of Veterinary and Animal Sciences
Volume	28
Issue number	3
State	Published - 2004
Externally published	Yes

Keywords

Conjugation
Exopolysaccharide
Lactococcus lactis
Plasmid

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title = "Conjugal transfer and stability of the plasmids determining exopolysaccharide production in Lactococcus lactis strains",

abstract = "A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 × 10-2 - 2.8 × 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 × 10-3 - 4.5 × 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.",

keywords = "Conjugation, Exopolysaccharide, Lactococcus lactis, Plasmid",

author = "Ela Ko{\c c}er and {\c C}aǧla T{\"u}kel and Mustafa Ak{\c c}elik",

year = "2004",

language = "English",

volume = "28",

pages = "481--487",

journal = "Turkish Journal of Veterinary and Animal Sciences",

issn = "1300-0128",

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}

TY - JOUR

T1 - Conjugal transfer and stability of the plasmids determining exopolysaccharide production in Lactococcus lactis strains

AU - Koçer, Ela

AU - Tükel, Çaǧla

AU - Akçelik, Mustafa

PY - 2004

Y1 - 2004

N2 - A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 × 10-2 - 2.8 × 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 × 10-3 - 4.5 × 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.

AB - A 27.2 kb plasmid in Lactococcus lactis subsp. lactis ELL23, a 32.7 kb plasmid in L. lactis subsp. cremoris ELC5 and a 31.1 kb plasmid in L. lactis subsp. cremoris ELC19 were found to determine exopolysaccharide production (Eps+) and lactose fermentation (Lac+) abilities at the end of conjugal transfer studies. Conjugal transfer frequencies (per donor cell) and stability rates of Lac+/Eps+ plasmids were determined as 1.0 × 10-2 - 2.8 × 10-3 and 80-96%, respectively, after conjugal matings between the aggregative (Agg+) wild type strains and Agg+ recipient strain L. lactis subsp. lactis LLT40. On the other hand, conjugal transfer frequencies and stability rates of Lac+/Eps+ plasmids occurred as 1.5 × 10-3 - 4.5 × 10-4 and 65-80%, respectively, after conjugal matings between the same donor strains and non-aggregative (Agg-) recipient strain L. lactis subsp. lactis ELL42-6.

KW - Conjugation

KW - Exopolysaccharide

KW - Lactococcus lactis

KW - Plasmid

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M3 - Article

AN - SCOPUS:3242798801

SN - 1300-0128

VL - 28

SP - 481

EP - 487

JO - Turkish Journal of Veterinary and Animal Sciences

JF - Turkish Journal of Veterinary and Animal Sciences

IS - 3

ER -

Conjugal transfer and stability of the plasmids determining exopolysaccharide production in Lactococcus lactis strains

Abstract

Keywords

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