TY - JOUR
T1 - Comparative genomic hybridization analysis detects frequent, often high- level, overrepresentation of DNA sequences at 3q, 5p, 7p, and 8q in human non-small cell lung carcinomas
AU - Balsara, Binaifer R.
AU - Sonoda, Gonosuke
AU - Du Manoir, Stanislas
AU - Siegfried, Jill M.
AU - Gabrielson, Edward
AU - Testa, Joseph R.
PY - 1997/6/1
Y1 - 1997/6/1
N2 - Comparative genomic hybridization analysis was used to identify chromosomal imbalances in 20 non-small cell lung carcinoma (NSCLC) biopsies and cell lines. The chromosome arms most often overrepresented were 3q (85%), 5p (70%), 7p (65%), and 8q (65%), which were observed at high copy numbers in many cases. Other common overrepresented sites were 1q, 2p, and 20p. DNA sequence amplification was often observed, with the most frequent site being 3q26 (six cases). Other recurrent sites of amplification included 8q24, 3q13, 3q28-qter, 7q11.2, 8p11-12, 12p12, and 19q13.1-13.2. The most frequent underrepresented segment was 3p21 (50%); other recurrent sites of autosomal loss included 8p21-pter, 15q11.2-13, 5q11.2-15, 9p, 13q12-14, 17p, and 18q21- qter. These regions of copy number decreases are also common sites of allelic loss, further implicating these sites as locations of tumor suppressor genes. Although some of the overrepresented segments harbor known or suspected oncogenes/growth-regulatory genes, we have identified 3q and 5p as new sites that are very frequently overrepresented in NSCLC. These findings could represent entry points for the identification of novel amplified DNA sequences that may contribute to the development or progression of NSCLC.
AB - Comparative genomic hybridization analysis was used to identify chromosomal imbalances in 20 non-small cell lung carcinoma (NSCLC) biopsies and cell lines. The chromosome arms most often overrepresented were 3q (85%), 5p (70%), 7p (65%), and 8q (65%), which were observed at high copy numbers in many cases. Other common overrepresented sites were 1q, 2p, and 20p. DNA sequence amplification was often observed, with the most frequent site being 3q26 (six cases). Other recurrent sites of amplification included 8q24, 3q13, 3q28-qter, 7q11.2, 8p11-12, 12p12, and 19q13.1-13.2. The most frequent underrepresented segment was 3p21 (50%); other recurrent sites of autosomal loss included 8p21-pter, 15q11.2-13, 5q11.2-15, 9p, 13q12-14, 17p, and 18q21- qter. These regions of copy number decreases are also common sites of allelic loss, further implicating these sites as locations of tumor suppressor genes. Although some of the overrepresented segments harbor known or suspected oncogenes/growth-regulatory genes, we have identified 3q and 5p as new sites that are very frequently overrepresented in NSCLC. These findings could represent entry points for the identification of novel amplified DNA sequences that may contribute to the development or progression of NSCLC.
KW - Biopsy
KW - Carcinoma, Non-Small-Cell Lung/genetics
KW - Chromosomes, Human, Pair 3
KW - Chromosomes, Human, Pair 5
KW - Chromosomes, Human, Pair 7
KW - Chromosomes, Human, Pair 8
KW - DNA, Neoplasm/metabolism
KW - Female
KW - Gene Amplification
KW - Humans
KW - Lung Neoplasms/genetics
KW - Male
KW - Nucleic Acid Hybridization
KW - Sequence Analysis, DNA
KW - Tumor Cells, Cultured
UR - http://www.scopus.com/inward/record.url?scp=0030923882&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1997XB75900010&DestLinkType=FullRecord&DestApp=WOS
M3 - Article
C2 - 9187106
SN - 0008-5472
VL - 57
SP - 2116
EP - 2120
JO - Cancer Research
JF - Cancer Research
IS - 11
ER -