Abstract
The ability to profile expression levels of a large number of mRNAs and microRNAs (miRNAs) within the same sample, using a single assay method, would facilitate investigations of miRNA effects on mRNA abundance and streamline biomarker screening across multiple RNA classes. A protocol is described for reverse transcription of long RNA and miRNA targets, followed by preassay amplification of the pooled cDNAs and quantitative PCR (qPCR) detection for a mixed panel of candidate RNA biomarkers. The method provides flexibility for designing custom target panels, is robust over a range of input RNA amounts, and demonstrated a high assay success rate.
Original language | English |
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Pages (from-to) | 75-83 |
Number of pages | 9 |
Journal | Journal of Biomolecular Techniques |
Volume | 27 |
Issue number | 2 |
DOIs | |
State | Published - Jul 2016 |
Keywords
- Adult
- Biomarkers/blood
- Case-Control Studies
- Gene Expression Profiling/instrumentation
- Humans
- MicroRNAs/blood
- Microfluidic Analytical Techniques
- RNA, Messenger/blood
- Reverse Transcriptase Polymerase Chain Reaction