TY - JOUR
T1 - Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line
AU - Taguchi, Takahiro
AU - Cheng, George Z.
AU - Bell, Daphne W.
AU - Balsara, Binaifer
AU - Liu, Zemin
AU - Siegfried, Jill M.
AU - Testa, Joseph R.
PY - 1997/10
Y1 - 1997/10
N2 - Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.
AB - Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.
KW - Blotting, Southern
KW - Carcinoma, Non-Small-Cell Lung/genetics
KW - Chromosome Banding
KW - Chromosome Mapping
KW - Chromosomes, Human, Pair 10/genetics
KW - Chromosomes, Human, Pair 7/genetics
KW - Chromosomes, Human, Pair 8/genetics
KW - Chromosomes, Human, Pair 9/genetics
KW - DNA, Neoplasm/analysis
KW - Epidermal Growth Factor/genetics
KW - Gene Amplification/genetics
KW - Genes, myc/genetics
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - Lung Neoplasms/genetics
KW - Polymerase Chain Reaction
KW - Tumor Cells, Cultured
UR - http://www.scopus.com/inward/record.url?scp=0030886084&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=purepublist2023&SrcAuth=WosAPI&KeyUT=WOS:A1997XZ20900012&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M
DO - 10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M
M3 - Article
C2 - 9331573
SN - 1045-2257
VL - 20
SP - 208
EP - 212
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 2
ER -