TY - JOUR
T1 - Circulating tumor cell and cell-free RNA capture and expression analysis identify platelet-associated genes in metastatic lung cancer
AU - Beck, Tim N.
AU - Boumber, Yanis A.
AU - Aggarwal, Charu
AU - Pei, Jianming
AU - Thrash-Bingham, Catherine
AU - Fittipaldi, Patricia
AU - Vlasenkova, Ramillya
AU - Rao, Chandra
AU - Borghaei, Hossein
AU - Cristofanilli, Massimo
AU - Mehra, Ranee
AU - Serebriiskii, Ilya
AU - Alpaugh, R. Katherine
N1 - Publisher Copyright:
© 2019 The Author(s).
PY - 2019/6/19
Y1 - 2019/6/19
N2 - Background: Circulating tumor cells (CTC) and plasma cell-free RNA (cfRNA) can serve as biomarkers for prognosis and treatment response in lung cancer. One barrier to the selected or routine use of CTCs and plasma cfRNA in precision oncology is the limited quantity of both, and CTCs are only seen in metastatic disease. As capture of CTCs and plasma cfRNA presents an opportunity to monitor and assess malignancies without invasive procedures, we compared two methods for CTC capture and identification, and profiled mRNA from CTCs and plasma cfRNA to identify potential tumor-associated biomarkers. Methods: Peripheral blood was collected from ten patients with small cell lung cancer (SCLC), ten patients with non-small cell lung cancer (NSCLC) and four healthy volunteers. Two methods were used for CTC capture: the standard epithelial cell adhesion molecule (EpCam) CellSearch kit (unicapture) and EpCAM plus HER2, EGFR and MUC-1 specific combined ferrofluid capture (quadcapture). For the quadcapture, anti-cytokeratin 7 (CK7) was additionally used to assist in CTC identification. NanoString analysis was performed on plasma cfRNA and on mRNA from combined ferrofluid isolated CTCs. Expression data was analyzed using STRING and Reactome. Results: Unicapture detected CTCs in 40% of NSCLC and 60% of SCLC; whereas, quadcapture/CK7 identified CTCs in 20% of NSCLC and 80% of SCLC. Bioinformatic analysis of NanoString data identified high expression of a platelet factor 4 (PF4)-related group of transcripts. Conclusions: Quadcapture ferrofluid reagent did not significantly improve CTC capture efficacy. NanoString analysis based on CTC and plasma cfRNA data highlighted an intriguing PF-4-centric network in patients with metastatic lung cancer.
AB - Background: Circulating tumor cells (CTC) and plasma cell-free RNA (cfRNA) can serve as biomarkers for prognosis and treatment response in lung cancer. One barrier to the selected or routine use of CTCs and plasma cfRNA in precision oncology is the limited quantity of both, and CTCs are only seen in metastatic disease. As capture of CTCs and plasma cfRNA presents an opportunity to monitor and assess malignancies without invasive procedures, we compared two methods for CTC capture and identification, and profiled mRNA from CTCs and plasma cfRNA to identify potential tumor-associated biomarkers. Methods: Peripheral blood was collected from ten patients with small cell lung cancer (SCLC), ten patients with non-small cell lung cancer (NSCLC) and four healthy volunteers. Two methods were used for CTC capture: the standard epithelial cell adhesion molecule (EpCam) CellSearch kit (unicapture) and EpCAM plus HER2, EGFR and MUC-1 specific combined ferrofluid capture (quadcapture). For the quadcapture, anti-cytokeratin 7 (CK7) was additionally used to assist in CTC identification. NanoString analysis was performed on plasma cfRNA and on mRNA from combined ferrofluid isolated CTCs. Expression data was analyzed using STRING and Reactome. Results: Unicapture detected CTCs in 40% of NSCLC and 60% of SCLC; whereas, quadcapture/CK7 identified CTCs in 20% of NSCLC and 80% of SCLC. Bioinformatic analysis of NanoString data identified high expression of a platelet factor 4 (PF4)-related group of transcripts. Conclusions: Quadcapture ferrofluid reagent did not significantly improve CTC capture efficacy. NanoString analysis based on CTC and plasma cfRNA data highlighted an intriguing PF-4-centric network in patients with metastatic lung cancer.
KW - Biomarkers, Tumor/genetics
KW - Carcinoma, Non-Small-Cell Lung/genetics
KW - Cell-Free Nucleic Acids/blood
KW - Epithelial Cell Adhesion Molecule/blood
KW - Humans
KW - Lung Neoplasms/genetics
KW - Neoplastic Cells, Circulating/metabolism
KW - Platelet Factor 4/blood
KW - Prognosis
KW - Small Cell Lung Carcinoma/genetics
UR - http://www.scopus.com/inward/record.url?scp=85067603297&partnerID=8YFLogxK
U2 - 10.1186/s12885-019-5795-x
DO - 10.1186/s12885-019-5795-x
M3 - Article
C2 - 31215484
AN - SCOPUS:85067603297
SN - 1471-2407
VL - 19
SP - 603
JO - Bmc Cancer
JF - Bmc Cancer
IS - 1
M1 - 603
ER -