Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors

Florina Olaru, Liselotte E. Jensen

Research output: Contribution to journalLetterpeer-review

54 Scopus citations

Abstract

Keratinocytes in the skin play an important role in innate immune responses by secreting chemokines. This study aimed to determine if keratinocyte cell lines can be used for studies of innate immune mechanisms. Human primary keratinocytes and the HaCaT, CCD 1106 KERTr (KERTr) and HEK001 cell lines were treated with a panel of Toll-like receptor (TLR)-ligands. Expression of IL-8, CCL20, CXCL9 and CXCL10 was determined. All three cell lines expressed TLR1-6 and TLR9. KERTr cells responded to the same TLR-ligands as primary keratinocytes. Overall HEK001 responded similarly, but appeared to be relatively more sensitive to flagellin. This was in agreement with increased expression of TLR5. The expression profiles were most distinct in HaCaT cells. Furthermore, our data confirm and extend previously reported TLR7 and TLR8 independent IL-8 secretion by keratinocytes after Imiquimod treatment. The different cell lines represent complementary tools for molecular studies of innate immunity of the skin.

Original languageEnglish
Pages (from-to)e314-e316
JournalExperimental Dermatology
Volume19
Issue number8
DOIs
StatePublished - Aug 2010
Externally publishedYes

Keywords

  • Chemokines
  • Human
  • Innate immunity of the skin
  • Keratinocytes
  • TLRs

Fingerprint

Dive into the research topics of 'Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors'. Together they form a unique fingerprint.

Cite this