@inbook{d2de805f1d0944b0ae4ca34fea1e5dec,
title = "Cell-based adhesion assays for isolation of snake venom{\textquoteright}s integrin antagonists",
abstract = "Snake venoms could lead to the development of new drugs to treat a range of life-threatening conditions like cardiovascular diseases. Most snake venoms contain a large variety of lethal toxins as well as anti-adhesive proteins such as disintegrins, which have evolved from the harmless compounds ADAMs (proteins with a disintegrin and a metalloprotease domain) and C-type lectin proteins which disturb connective tissue and cell-matrix interaction. These anti-adhesive proteins target and block integrin receptors and disrupt normal biological processes in snakes{\textquoteright} prey such as connective tissue physiology and blood clotting. This chapter provides the experimental details of a practical, cell-based adhesion protocol to help identify and isolate disintegrins and C-type lectin proteins from snake venoms, important tools in integrin research and lead compounds for drug discovery.",
keywords = "Animals, Biological Assay, Blood Coagulation/drug effects, Cell Adhesion/physiology, Disintegrins/antagonists & inhibitors, Humans, Lectins/chemistry, Snake Venoms/analysis",
author = "Philip Lazarovici and Cezary Marcinkiewicz and Lelkes, {Peter I.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-4939-9845-6_11",
language = "English",
volume = "2068",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press Inc.",
pages = "205--223",
booktitle = "Methods in Molecular Biology",
address = "United States",
}
