Abstract
We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6-20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain.
Original language | English |
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Pages (from-to) | 291-294 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 7 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2010 |