Atomic accuracy in predicting and designing noncanonical RNA structure

Rhiju Das, John Karanicolas, David Baker

Research output: Contribution to journalArticlepeer-review

292 Scopus citations

Abstract

We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6-20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain.

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalNature Methods
Volume7
Issue number4
DOIs
StatePublished - Apr 2010

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