Abstract
We undertook a prospective flow and static cytometric study of proliferative activity in 74 malignant, borderline and benign ovarian neoplasms. Proliferative activity as assessed by S phase fraction (SPF), and immunostaining of tissue sections with Ki-67 antibody was compared with prognostically important clinicopathologic features. Malignant neoplasms had higher median percentage Ki-67 staining (27.6%) than borderline (12.3%) and benign (2.7%) tumors (P < 0.05). Percentage Ki-67 staining correlated with SPF, DNA index, architectural grade, nucleolar grade, and mitotic count in malignant tumors; with nucleolar grade in benign tumors and with none of these variables in borderline tumors. Similarly, malignant neoplasms had a higher median SPF (11.5%) than borderline (3.4%) and benign (2.9%) neoplasms (P < 0.05). In malignant neoplasms, SPF correlated with percentage Ki-67 staining, DNA index, age, and stage, but with none of these in borderline or benign neoplasms.
Original language | English |
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Pages (from-to) | 699-706 |
Number of pages | 8 |
Journal | American Journal of Pathology |
Volume | 141 |
Issue number | 3 |
State | Published - Sep 1992 |
Keywords
- Aged
- Cell Division
- Cell Nucleus/metabolism
- Cytological Techniques
- DNA/genetics
- Female
- Flow Cytometry
- Humans
- Image Processing, Computer-Assisted
- Immunoenzyme Techniques
- Ki-67 Antigen
- Middle Aged
- Nuclear Proteins/analysis
- Ovarian Neoplasms/metabolism
- Ploidies
- Prognosis
- S Phase
- Staining and Labeling