Assessment of proliferative activity in ovarian neoplasms by flow and static cytometry: Correlation with prognostic features

Phyllis C. Huettner, David S. Weinberg, Janice M. Lage

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We undertook a prospective flow and static cytometric study of proliferative activity in 74 malignant, borderline and benign ovarian neoplasms. Proliferative activity as assessed by S phase fraction (SPF), and immunostaining of tissue sections with Ki-67 antibody was compared with prognostically important clinicopathologic features. Malignant neoplasms had higher median percentage Ki-67 staining (27.6%) than borderline (12.3%) and benign (2.7%) tumors (P < 0.05). Percentage Ki-67 staining correlated with SPF, DNA index, architectural grade, nucleolar grade, and mitotic count in malignant tumors; with nucleolar grade in benign tumors and with none of these variables in borderline tumors. Similarly, malignant neoplasms had a higher median SPF (11.5%) than borderline (3.4%) and benign (2.9%) neoplasms (P < 0.05). In malignant neoplasms, SPF correlated with percentage Ki-67 staining, DNA index, age, and stage, but with none of these in borderline or benign neoplasms.

Original languageEnglish
Pages (from-to)699-706
Number of pages8
JournalAmerican Journal of Pathology
Volume141
Issue number3
StatePublished - Sep 1992

Keywords

  • Aged
  • Cell Division
  • Cell Nucleus/metabolism
  • Cytological Techniques
  • DNA/genetics
  • Female
  • Flow Cytometry
  • Humans
  • Image Processing, Computer-Assisted
  • Immunoenzyme Techniques
  • Ki-67 Antigen
  • Middle Aged
  • Nuclear Proteins/analysis
  • Ovarian Neoplasms/metabolism
  • Ploidies
  • Prognosis
  • S Phase
  • Staining and Labeling

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