Anti-human embryonic stem cell monoclonal antibody hesca-2 binds to a glycan epitope commonly found on carcinomas

Mohamed G. Shoreibah; Crystal L. Jackson; Paul W. Price; Richard Meagher; Andrew K. Godwin; Qi Cai; Jeffrey C. Gildersleeve

doi:10.1089/scd.2010.0172

Anti-human embryonic stem cell monoclonal antibody hesca-2 binds to a glycan epitope commonly found on carcinomas

Mohamed G. Shoreibah, Crystal L. Jackson, Paul W. Price, Richard Meagher, Andrew K. Godwin, Qi Cai, Jeffrey C. Gildersleeve

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Hesca-2, a monoclonal antibody (mAb) IgM raised to the human embryonic stem cell (hESC) line BG-01v, binds with high affinity (nM) to the disaccharide epitope (Galβ1-3GlcNAc) on a glycan microarray. This epitope was expressed on pluripotent progenitor hESCs in culture, but not in various differentiated cells derived from hESC based on immunofluorescence microscopy. Hesca-2 stains a limited subset of cells in adult human tissues (eg, esophagus and breast). This mAb also crossreacts in immunofluorescence microscopy studies with several human ovarian cancer cell lines and is cytotoxic to them based on the release of cytosolic enzyme lactate dehydrogenase into the media. Hesca-2 immunohistochemically stained tissue from a number of human tumors, including ovary, breast, colon, and esophageal cancer. These data suggest that Hesca-2 recognizes a surface marker found both in stem cells and certain cancer cells.

Original language	English
Pages (from-to)	515-525
Number of pages	11
Journal	Stem Cells and Development
Volume	20
Issue number	3
DOIs	https://doi.org/10.1089/scd.2010.0172
State	Published - Mar 1 2011

Keywords

Animals
Antibodies, Monoclonal, Murine-Derived/metabolism
Antibody Affinity
Antigens, Surface/immunology
Biomarkers, Tumor/metabolism
Cell Line
Cross Reactions
Disaccharides/metabolism
Embryonic Stem Cells/immunology
Epitopes
Humans
L-Lactate Dehydrogenase/metabolism
Mice
Microarray Analysis
Microscopy, Fluorescence
Neoplasms/metabolism
Neoplastic Stem Cells/metabolism
Polysaccharides/metabolism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/scd.2010.0172

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title = "Anti-human embryonic stem cell monoclonal antibody hesca-2 binds to a glycan epitope commonly found on carcinomas",

abstract = "Hesca-2, a monoclonal antibody (mAb) IgM raised to the human embryonic stem cell (hESC) line BG-01v, binds with high affinity (nM) to the disaccharide epitope (Galβ1-3GlcNAc) on a glycan microarray. This epitope was expressed on pluripotent progenitor hESCs in culture, but not in various differentiated cells derived from hESC based on immunofluorescence microscopy. Hesca-2 stains a limited subset of cells in adult human tissues (eg, esophagus and breast). This mAb also crossreacts in immunofluorescence microscopy studies with several human ovarian cancer cell lines and is cytotoxic to them based on the release of cytosolic enzyme lactate dehydrogenase into the media. Hesca-2 immunohistochemically stained tissue from a number of human tumors, including ovary, breast, colon, and esophageal cancer. These data suggest that Hesca-2 recognizes a surface marker found both in stem cells and certain cancer cells.",

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author = "Shoreibah, {Mohamed G.} and Jackson, {Crystal L.} and Price, {Paul W.} and Richard Meagher and Godwin, {Andrew K.} and Qi Cai and Gildersleeve, {Jeffrey C.}",

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doi = "10.1089/scd.2010.0172",

language = "English",

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AU - Shoreibah, Mohamed G.

AU - Jackson, Crystal L.

AU - Price, Paul W.

AU - Meagher, Richard

AU - Godwin, Andrew K.

AU - Cai, Qi

AU - Gildersleeve, Jeffrey C.

PY - 2011/3/1

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N2 - Hesca-2, a monoclonal antibody (mAb) IgM raised to the human embryonic stem cell (hESC) line BG-01v, binds with high affinity (nM) to the disaccharide epitope (Galβ1-3GlcNAc) on a glycan microarray. This epitope was expressed on pluripotent progenitor hESCs in culture, but not in various differentiated cells derived from hESC based on immunofluorescence microscopy. Hesca-2 stains a limited subset of cells in adult human tissues (eg, esophagus and breast). This mAb also crossreacts in immunofluorescence microscopy studies with several human ovarian cancer cell lines and is cytotoxic to them based on the release of cytosolic enzyme lactate dehydrogenase into the media. Hesca-2 immunohistochemically stained tissue from a number of human tumors, including ovary, breast, colon, and esophageal cancer. These data suggest that Hesca-2 recognizes a surface marker found both in stem cells and certain cancer cells.

AB - Hesca-2, a monoclonal antibody (mAb) IgM raised to the human embryonic stem cell (hESC) line BG-01v, binds with high affinity (nM) to the disaccharide epitope (Galβ1-3GlcNAc) on a glycan microarray. This epitope was expressed on pluripotent progenitor hESCs in culture, but not in various differentiated cells derived from hESC based on immunofluorescence microscopy. Hesca-2 stains a limited subset of cells in adult human tissues (eg, esophagus and breast). This mAb also crossreacts in immunofluorescence microscopy studies with several human ovarian cancer cell lines and is cytotoxic to them based on the release of cytosolic enzyme lactate dehydrogenase into the media. Hesca-2 immunohistochemically stained tissue from a number of human tumors, including ovary, breast, colon, and esophageal cancer. These data suggest that Hesca-2 recognizes a surface marker found both in stem cells and certain cancer cells.

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KW - Antibody Affinity

KW - Antigens, Surface/immunology

KW - Biomarkers, Tumor/metabolism

KW - Cell Line

KW - Cross Reactions

KW - Disaccharides/metabolism

KW - Embryonic Stem Cells/immunology

KW - Epitopes

KW - Humans

KW - L-Lactate Dehydrogenase/metabolism

KW - Mice

KW - Microarray Analysis

KW - Microscopy, Fluorescence

KW - Neoplasms/metabolism

KW - Neoplastic Stem Cells/metabolism

KW - Polysaccharides/metabolism

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Anti-human embryonic stem cell monoclonal antibody hesca-2 binds to a glycan epitope commonly found on carcinomas

Abstract

Keywords

UN SDGs

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