Analysis of PTP1B sumoylation

Sayanti Saha; Jonathan Chernoff

doi:10.1016/j.ymeth.2013.09.012

Analysis of PTP1B sumoylation

Sayanti Saha, Jonathan Chernoff

Cancer Signaling and Microenvironment (CSM)

Fox Chase Cancer Center

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well.

Original language	English
Pages (from-to)	201-206
Number of pages	6
Journal	Methods
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2013.09.012
State	Published - Jan 15 2014

Keywords

Phosphorylation
Posttranslational modification
Protein tyrosine phosphatases
Signal transduction
Sumoylation

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10.1016/j.ymeth.2013.09.012

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title = "Analysis of PTP1B sumoylation",

abstract = "Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well.",

keywords = "Phosphorylation, Posttranslational modification, Protein tyrosine phosphatases, Signal transduction, Sumoylation",

author = "Sayanti Saha and Jonathan Chernoff",

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N2 - Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well.

AB - Signaling enzymes are typically regulated by a variety of post-translational modifications. One such enzyme, protein tyrosine phosphatase (PTP) 1B, which plays an important role in metabolic and growth signaling, has recently been shown to be inhibited by posttranslational modifications by ubiquitin-related proteins of the SUMO family. We have adapted several approaches to analyzing the sites and effects of sumoylation of PTP1B in cells and in vitro. The principal outline of the assays should be applicable to other enzymes as well.

KW - Phosphorylation

KW - Posttranslational modification

KW - Protein tyrosine phosphatases

KW - Signal transduction

KW - Sumoylation

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JO - Methods

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Analysis of PTP1B sumoylation

Abstract

Keywords

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